Cas13d-mediated multiplex RNA targeting confers a broad-spectrum resistance against RNA viruses in potato

文献类型： 外文期刊

第一作者： Zhan, Xiaohui

作者： Zhan, Xiaohui;Liu, Wenting;Zhang, Fengjuan;Zhang, Jiang;Nie, Bihua;Zhang, Jiang

作者机构：

期刊名称：COMMUNICATIONS BIOLOGY （ 影响因子：5.9； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 6 卷 1 期

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收录情况： SCI

摘要： CRISPR/Cas13d coupled with an endogenous tRNA-processing system can be used to engineer resistance to multiple RNA viruses in potato. CRISPR-Cas systems endow the bacterial and archaeal species with adaptive immune mechanisms to fend off invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13d has been harnessed to confer the protection against RNA viruses in diverse eukaryotic species. However a vast number of different viruses can potentially infect the same host plant resulting in mixed infection, thus necessitating the generation of crops with broad-spectrum resistance to multiple viruses. Here we report the repurposing of CRISPR/Cas13d coupled with an endogenous tRNA-processing system (polycistronic tRNA-gRNA, PTG) to target the multiple potato RNA viruses. Expression of Cas13d and four different gRNAs were observed in transgenic potato lines expressing the Cas13d/PTG construct. We show that the Cas13d/PTG transgenic plants exhibit resistance to either PVY, PVS, PVX or PLRV alone or two/three viruses simultaneously by reducing viral accumulation in plant cells. In sum, our findings provide an efficient strategy for engineering crops that can simultaneously resist infection by multiple RNA viruses.

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