SY18 & UDelta;L60L: a new recombinant live attenuated African swine fever virus with protection against homologous challenge

文献类型： 外文期刊

第一作者： Yang, Jinjin

作者： Yang, Jinjin;Li, Qixuan;Qian, Aidong;Yang, Jinjin;Zhu, Rongnian;Zhang, Yanyan;Yue, Huixian;Miao, Faming;Chen, Teng;Mi, Lijuan;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang;Yang, Jinjin;Zhu, Rongnian;Zhang, Yanyan;Yue, Huixian;Miao, Faming;Chen, Teng;Mi, Lijuan;Zhang, Fei;Zhang, Shoufeng;Hu, Rongliang;Fan, Jiaqi;Zhou, Xintao

作者机构：

关键词： African swine fever virus; L60L; deleted; attenuated virus; recombinant virus

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： IntroductionAfrican swine fever (ASF) is an acute and highly contagious disease and its pathogen, the African swine fever virus (ASFV), threatens the global pig industry. At present, management of ASF epidemic mainly relies on biological prevention and control methods. Moreover, due to the large genome of ASFV, only half of its genes have been characterized in terms of function. MethodsHere, we evaluated a previously uncharacterized viral gene, L60L. To assess the function of this gene, we constructed a deletion strain (SY18 & UDelta;L60L) by knocking out the L60L gene of the SY18 strain. To evaluate the growth characteristics and safety of the SY18 & UDelta;L60L, experiments were conducted on primary macrophages and pigs, respectively. ResultsThe results revealed that the growth trend of the recombinant strain was slower than that of the parent strain in vitro. Additionally, 3/5 (60%) pigs intramuscularly immunized with a 105 50% tissue culture infectious dose (TCID50) of SY18 & UDelta;L60L survived the 21-day observation period. The surviving pigs were able to protect against the homologous lethal strain SY18 and survive. Importantly, there were no obvious clinical symptoms or viremia. DiscussionThese results suggest that L60L could serve as a virulence- and replication-related gene. Moreover, the SY18 & UDelta;L60L strain represents a new recombinant live-attenuated ASFV that can be employed in the development of additional candidate vaccine strains and in the elucidation of the mechanisms associated with ASF infection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序