Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
文献类型： 外文期刊
第一作者： Jin, Q. Y.
作者： Jin, Q. Y.;Yang, J. F.;Teng, M.;Chai, S. J.;Xing, G. X.;Zhang, G. P.;Feng, L. L.;Wang, Y. B.;Li, P.
作者机构：
关键词： porcine circovirus type 2 (PCV2); screening of MAbs; paper test
期刊名称：POLISH JOURNAL OF VETERINARY SCIENCES （ 影响因子：0.859； 五年影响因子：1.008 ）
ISSN： 1505-1773
年卷期： 2022 年 25 卷 1 期
页码：
收录情况： SCI
摘要： A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
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