Abnormalities in mitochondrial energy metabolism induced by cryopreservation negatively affect goat sperm motility

文献类型： 外文期刊

第一作者： Zang, Shengqin

作者： Zang, Shengqin;Zou, Shuqi;Chen, Xiangyi;Pan, Bo;Ning, Ao;Qin, Jianpeng;Wei, Yaozong;Du, Kunlin;Ye, Jiangfeng;Zhou, Guangbin;Liang, Qiuxia;Fang, Yi;Qiongla, Tianzeng;Cirenlamu, Guangbin;Song, Tianzeng;Song, Tianzeng

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关键词： goat sperm; cryopreservation; sperm motility; energy metabolism; metabolomics

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

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年卷期： 2025 年 11 卷

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收录情况： SCI

摘要： The motility of sperm decreases following cryopreservation, which is closely associated with mitochondrial function. However, the alterations in mitochondrial metabolism after sperm freezing in goats remain unclear. This experiment aimed to investigate the impact of ultra-low temperature freezing on goat sperm's mitochondrial energy metabolism and its potential correlation with sperm motility. The results revealed that goat sperm exhibited mitochondrial vacuolization, reduced matrix density, and significantly decreased levels of high-membrane potential mitochondria and adenosine triphosphate content, accompanied by a substantial increase in reactive oxygen species levels, ultimately leading to a significant decline in sperm viability. Further investigations unveiled that energy-related differential metabolites (capric acid, creatine, and D-glucosamine-6-phosphate) and differential metabolites with antioxidant effects (saikosaponin A, probucol, and cholesterol sulfate) were significantly downregulated. In addition, the activity of key rate-limiting enzymes involved in very long-chain fatty acid biosynthesis and beta-oxidation-specifically acetyl-CoA carboxylase, fatty acid synthase, and carnitine palmitoyltransferase I related to capric acid metabolism-was considerably reduced. Furthermore, supplementation of differential metabolite capric acid (500 mu M) significantly enhanced the motility of frozen-thawed goat sperm. These findings indicated that the mitochondrial ultrastructure of goat sperm is damaged and energy metabolism becomes abnormal after cryopreservation, potentially affecting sperm viability. The addition of different metabolites such as capric acid to the freezing extender can alleviate the decrease in sperm motility induced by cryopreservation.

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