Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity

文献类型： 外文期刊

第一作者： Xu, Lian

作者： Xu, Lian;Zheng, Mei-Xia;Zhu, Yu-Jing;Fu, Bai-Wen;Shi, Yan

作者机构：

关键词： Cry2Ab; Protein engineering; Oligomerization; Pore-forming activity; Insecticidal activity

期刊名称：AMB EXPRESS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2191-0855

年卷期： 2024 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC50 (0.129 mu g/cm(2)) against P. xylostella when compared to wild-type Cry2Ab (0.267 mu g/cm(2)). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed higher oligomerization and pore-formation activities, which was consistence with its increased insecticidal activity. The current study demonstrated that the insecticidal activity of Cry2Ab toxin could be assessed using oligomerization and pore-formation activities, and the screened variant L183I with improved activity might contribute to Cry2Ab toxin's future application.

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