An Integrative Analysis of the Transcriptome and Proteome of Rice Grain Chalkiness Formation Under High Temperature

文献类型： 外文期刊

第一作者： Zhao, Shaolu

作者： Zhao, Shaolu;Cao, Ruijie;Zhong, Min;Zhao, Fengli;Jiao, Guiai;Chen, Pengfei;Li, Xinwei;Duan, Yingqing;Li, Xiaoxue;Tang, Shaoqing;Ni, Shen;Hu, Peisong;Wei, Xiangjin;Zhao, Shaolu;Sun, Linhe;Zhuang, Dongying;Zhao, Fengli

作者机构：

关键词： rice quality; high temperature; starch biosynthesis; transcriptome; proteome

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 23 期

页码：

收录情况： SCI

摘要： Exposure to high temperatures can impair the grain-filling process in rice (Oryza sativa L.), potentially leading to the formation of chalky endosperm, but the molecular regulation mechanism remains largely elusive. Here, we reported that high-temperature (HT) stress (day/night, 35 degrees C/30 degrees C) reduces both the grain-filling rate and grain weight of Ningjing 1 variety compared to normal temperatures (NT, day/night, 28 degrees C/23 degrees C). Grains under HT stress exhibited an opaque, milky-white appearance, alongside significant alterations in starch physicochemical properties. An integrated transcriptomic analysis of grains under HT revealed up-regulation of genes related to defense mechanisms and oxidoreductase activity, while genes involved in sucrose and starch synthesis were down-regulated, and alpha-amylase genes were up-regulated. Proteomic analysis of grains under HT echoed this pattern. These results demonstrate that high temperature during the grain-filling stage significantly increases rice chalkiness by down-regulating genes related to sucrose and starch synthesis, while up-regulating those involved in starch degradation.

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