A clinical Pseudomonas juntendi strain with bla(IMP-1) carried by an integrative and conjugative element in China

文献类型： 外文期刊

第一作者： Zheng, Lin

作者： Zheng, Lin;Liu, Mingwei;Chen, Ping;Zheng, Lin;Zhu, Lingwei;Lu, Gejin;Guan, Jiayao;Liu, Mingwei;Jing, Jie;Sun, Shiwen;Wang, Ying;Sun, Yang;Ji, Xue;Jiang, Bowen;Chen, Jun;Liu, Jun;Guo, Xuejun;Zhang, Xinfang

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关键词： Pseudomonas juntendi; integrative and conjugative; carbapenem-resistant; reorganization; bla(IMP-1)

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

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收录情况： SCI

摘要： ObjectiveTo precisely determine the species of a carbapenem-resistant Pseudomonas strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) 1276 formation mechanism. MethodsSingle-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE 1276 were aligned. At the same time, the transfer ability of bla(IMP-1) and the antibiotic sensitivity of Pseudomonas juntendi 18091276 were tested. ResultsThis bacterium was P. juntendi, and its drug resistance mechanism is the capture of the accA4' gene cassette by the Tn402-like type 1 integron (IntI1-bla(IMP-1)) to form In1886 before its capture by the Delta Tn4662a-carrying ICE 1276. The acquisition of bla(IMP-1) confers carbapenem resistance to P. juntendi 18091276. ConclusionThe formation of bla(IMP-1)-carrying ICE 1276, its further integration into the chromosomes, and transposition and recombination of other elements promote bacterial gene accumulation and transmission.

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