Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway

文献类型： 外文期刊

第一作者： Yu, Siriguleng

作者： Yu, Siriguleng;Liu, Chaoqun;Lv, Hong;Guo, Zhijun;Yao, Hongqiang;Wen, Xin;Li, Ziyi;Yu, Wen;Niu, Yaru;Jin, Junjian;Yu, Siriguleng;Yao, Hongqiang;Xiu, Ying;Gao, Wa

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关键词： Bactrian camel; exosome; metabolism; miR-144-5p; PGC-1 alpha

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

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年卷期： 2025 年 12 卷

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收录情况： SCI

摘要： Introduction MiR-144-5p is differentially expressed in plasma exosomes from Bactrian camels of varying body sizes, with GO and KEGG analyses indicating that its target genes play crucial roles in lipid metabolism. PGC-1 alpha, confirmed as a key target through RNA pull-down and dual-luciferase reporter assays, is a significant regulator of this process. This study aims to investigate the impact of miR-144-5p on lipid metabolism in a mouse model of high-fat diet (HFD)-induced obesity to elucidate the mechanistic pathways involved.Methods Male C57BL/6 mice were assigned to groups and fed either a control diet or an HFD for 12 weeks, which was followed by a 3-week intervention with miR-144-5p. Various metabolic parameters and gene expressions were evaluated to determine the effects of this treatment.Results The treatment significantly reduced diet-induced adiposity and decreased inflammatory responses. Additionally, it inhibited PGC-1 alpha expression in the liver tissue of HFD-fed mice. There was an increase in CPT1 activity and fatty acid beta-oxidation rate, along with the downregulation of FASN mRNA expression and the upregulation of CPT1 and ACOX1 expression, promoting fatty acid oxidation.Discussion These findings suggest that miR-144-5p exerts anti-obesity effects by enhancing fatty acid oxidation, likely through modulation of the PGC-1 alpha/AMPK signaling pathway. This study provides new insights into potential miRNA-based therapeutic strategies for obesity and related metabolic disorders.

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