Isoorientin Affects Markers of Alzheimer's Disease via Effects on the Oral and Gut Microbiota in APP/PS1 Mice

文献类型： 外文期刊

第一作者： Zhang, Zhongbao

作者： Zhang, Zhongbao;Sun, Xiaorong;Wei, Jianhua;Wu, Zhongyi;Tan, Xiaoqin;Li, Qing X.;Tan, Xiaoqin

作者机构：

关键词： Alzheimer's; isoorientin; amyloid-beta; oral microbiota; gut microbiota

期刊名称：JOURNAL OF NUTRITION （ 影响因子：4.687； 五年影响因子：5.76 ）

ISSN： 0022-3166

年卷期： 2022 年 152 卷 1 期

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收录情况： SCI

摘要： Background: There is growing evidence of strong associations between the pathogenesis of Alzheimer's disease (AD) and dysbiotic oral and gut microbiota. Recent studies demonstrated that isoorientin (ISO) is anti-inflammatory and alleviates markers of AD, which were hypothesized to be mediated by the oral and gut microbiota. Objectives: We studied the effects of oral administration of ISO on AD-related markers and the oral and gut microbiota in mice. Methods: Eight-month-old amyloid precursor protein/presenilin-1 (AP) transgenic male mice were randomly allocated to 3 groups of 15 mice each: vehicle (AP) alone or with a low dose of ISO (AP + ISO-L; 25 mg/kg) or a high dose of ISO (AP + ISO-H; 50 mg/kg). Age-matched wild-type (WT) C57BL/6 male littermates were used as controls. The 4 groups were treated intragastrically with ISO or sterilized ultrapure water for 2 months. AD-related markers in the brain, serum, colon, and liver were analyzed with immunohistochemical and histochemical staining, Western blotting, and ELISA. Oral and gut microbiotas were analyzed using 16S ribosomal RNA gene sequencing. Results: The high-dose ISO treatment significantly decreased amyloid beta 42-positive deposition by 38.1% and 45.2% in the cortex and hippocampus, respectively, of AP mice (P < 0.05). Compared with the AP group, both ISO treatments reduced brain phospho-Tau, phosphor-p65, phosphor-inhibitor of NF-kappa B, and brain and serum LPS and TNF-alpha by 179%-72.5% and increased brain and serum IL-4 and IL-10 by 130%-210% in the AP + ISO-L and AP + ISO-H groups (P< 0.05). Abundances of 26, 25, and 23 microbial taxa in oral, fecal and cecal samples, respectively, were increased in both the AP + ISO-L and AP + ISO-H groups relative to the AP group (linear discriminant analysis (LDA) >3.0; P < 0.051]. Gramnegative bacteria, Alteromonas, Campylobacterales, and uncultured Bacteroidales bacterium were positively correlated (rho = 0.28-0.59; P < 0.05) with the LPS levels and responses of inflammatory cytokines. Conclusions: The microbiota-gut-brain axis is a potential mechanism by which ISO reduces AD-related markers in AP mice.

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