Assembling "Balloon-String" Colorimetric Probes through Click Chemistry and Hybridization Chain Reaction for Dual-Signal Amplification in Aptamer-Based Lateral Flow Assays

文献类型： 外文期刊

第一作者： Dong, Haowei

作者： Dong, Haowei;Zhang, Pengwei;Huang, Jingcheng;Guo, Zhen;Guo, Yemin;Sun, Xia;Wang, Haifang;Cai, Ziping;Darwish, Ibrahim A.

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.7； 五年影响因子：6.6 ）

ISSN： 0003-2700

年卷期： 2025 年 97 卷 19 期

页码：

收录情况： SCI

摘要： As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs are being developed for rapid detection. However, the sensitivity of conventional gold nanoparticles (AuNPs) aptamer-based LFAs is limited, which is a challenge to meet the detection requirements. This study proposed a dual amplification strategy of click chemistry and hybridization chain reaction (HCR) to construct a colorimetric probe to enhance the sensitivity. The nucleic acid nanostructure (HCR-ALK) constructed by HCR was used as a programming template to guide the click chemical reaction to induce the cross-linking of AuNPs-N3 and HCR-ALK to form a uniform and stable '' ballon-string '' colorimetric probe (HCR-AuNPs). This strategy achieved dual-signal amplification. Under optimal conditions, the limit of detection (LOD) of this LFA was 5.4 x 10-2 ng/mL. Compared with the conventional AuNPs aptamer-based LFAs, its LOD had been improved by 13.69-fold, and its color display time had been reduced to one-tenth. In specificity and stability experiments, this LFA showed a satisfactory performance. Moreover, it was successfully applied to the detection of procymidone in vegetable samples, with a recovery rate maintained between 93.44%-104.00%. Thus, this study provides a signal amplification strategy to address the issue of insufficient sensitivity in aptamer-based LFAs. This will offer new insights for the development of aptamer-based LFAs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序