Alpha-ketoglutaric acid mitigates the detrimental effects of soy antigenic protein on the intestinal health and growth performance of Mirror carp Cyprinus carpio

文献类型： 外文期刊

第一作者： Zhou, Zuliang

作者： Zhou, Zuliang;Zhao, Jianhua;Xu, Hong;Xu, Qiyou;Zhou, Zuliang;de Cruz, Clement R.;Wang, Liansheng

作者机构：

关键词： Soy antigen protein; Intestine; Metabolome; AKG; Carp

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2023 年 49 卷 5 期

页码：

收录情况： SCI

摘要： The study investigated the alleviated effects of Alpha-ketoglutaric acid (AKG) on the intestinal health of mirror carp (Cyprinus carpio Songpu) caused by soy antigenic protein. The diets were formulated from fishmeal (CON), 50% soybean meal (SBM), the mixture of glycinin and beta-conglycinin (11+ 7S) and adding 1% AKG in the 11 + 7S (AKG). Carp (similar to 4 g) in triplicate (30 fish per tank) was fed to apparent satiation thrice a day for six weeks. Compared with CON, SBM treatment resulted in significantly poor growth performance (P <0.05), whereas 11 +7S and AKG treatments were not significantly different from CON (P> 0.05). Gene expression of tumor necrosis factor (TNF-alpha) and interleukin-1 beta (IL1 beta) in proximal intestines (PI) and distal intestines (DI) were increased (P< 0.05), and transforming growth factor (TGF-beta) in PI and middle intestines (MI) was decreased (P <0.05) in both SBM and 11+ 7S. The caspase-3 in DI increased in SBM (P < 0.05) and the caspase-3 and caspase-9 in DI increased in 11 + 7S (P < 0.05); conversely, TGF-beta in PI and MI was increased, TNF-alpha and IL-1 beta in the MI, caspase-3, and caspase-9 in DI was decreased in AKG (P< 0.05). The TOR (target of rapamycin) in PI and MI, ACC in PI, MI and DI was decreased in SBM (P < 0.05), the AMPK in the PI and DI, TOR in PI, MI and DI, ACC in PI and DI, 4E-BP in DI was reduced in 11 +7S (P < 0.05). AMPK in the PI and DI, ACC in the PI and MI, TOR in PI, MI, and DI, 4E-BP in PI and DI was recovered by AKG supplementation (P <0.05). Lipids and lipid-like metabolism, organic acids and derivatives metabolism increased in AKG dietary treatment. In conclusion, AKG reduces the expression of intestinal inflammation and apoptosis pathway and changes glycerophospholipid metabolism and sphingolipid metabolism in the intestine of fish.

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