Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya

文献类型： 外文期刊

第一作者： Wu, Zhijiang

作者： Wu, Zhijiang;Huang, Lifang;Huang, Fengzhu;Lu, Guifeng;Wei, Shuotong;Liu, Chaoan;Deng, Haiyan;Liang, Guidong

作者机构：

关键词： Cactaceae; Dragon fruit; Edible ornamental fruit plant; Flower; Flower development; Gene expression; Hylocereus polyrhizus; Pitaya; Transcription factor; Transcriptional regulation; Transcriptome; Unigenes

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.826； 五年影响因子：3.511 ）

ISSN： 0717-3458

年卷期： 2022 年 58 卷

页码：

收录情况： SCI

摘要： Background: Pitaya is an important economic fruit worldwide due to its numerous health benefits. A systematic understanding of the mechanism underlying flower development is essential to obtain higher fruit yield and quality. However, the genetic mechanism of flower development is not yet investigated in pitaya. Herein, a transcriptome analysis was performed to determine the transcriptional changes during flower development in red-flesh pitaya by utilizing nine different stages of flower development. Result: A total of 95,412 unigenes were generated with a mean length of 913 nt, and N50 value of 1878 nt. Comparative transcriptomic analysis showed many differentially expressed genes (DEGs) among the flower growth stages. Furthermore, an array of key DEGs were enriched in hormone signaling, transcription, carbohydrate transport, and energy production pathways. In particular, indole-3-acetic acid, abscisic acid, ethylene-responsive transcription factor, constans-like, teosinte branched 1/cycloidea/proliferating cell nuclear antigen factor, apetala1-like, agamous-like MADS-box protein, sepallata, growth-regulating factor, putative axial regulator yabby, leafy/floricaula homolog, and MYB gene-associated transcription factors displayed altered expression, suggesting their critical roles in floral organ development of pitaya. Besides, genes related to sugar synthesis, transportation, and utilization mediate flower growth regulation in pitaya. Eleven genes were selected to perform qRT-PCR analysis to verify the results of the RNA sequencing. Conclusions: Our results provide important insights into the transcriptional regulation of pitaya flower development. These data resources will be a cornerstone for future research that aims at exploring the genetic control of flower development in pitaya. How to cite: Wu Z, Huang L, Huang F, et al. Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya. Electron J Biotechnol 2022;58 https://doi.org/10.1016/j.ejbt. (c) 2022 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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