Mutation of the Polygalacturonase Gene AcoPG3 Deferred Softening of Pineapple Fruit

文献类型： 外文期刊

第一作者： Shu, Haiyan

作者： Shu, Haiyan;Luan, Aiping;Wang, You;He, Junhu;Wei, Qing;Zhan, Rulin;Chang, Shenghe;Shu, Haiyan;Zhan, Rulin;Chang, Shenghe;Shu, Haiyan;Zhan, Rulin;Chang, Shenghe;Shu, Haiyan;Luan, Aiping;Wang, You;He, Junhu;Wei, Qing;Zhan, Rulin;Chang, Shenghe;Shu, Haiyan;Luan, Aiping;Wang, You;He, Junhu;Wei, Qing;Zhan, Rulin;Chang, Shenghe

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关键词： cell wall; pectin; shelf life; degradation; mutant

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

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年卷期： 2025 年 14 卷 5 期

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收录情况： SCI

摘要： A large number of pineapple (Ananas comosus) fruits are discarded in China every year due to softening. However, the underlying molecular mechanism is still unknown. AcoPG3 (GenBank accession number: XM020243935), a pineapple gene of polygalacturonase, was found to be the major gene responsible for the softening of pineapple fruit. Fruit of AcoPG3-overexpressing tomato (Solanum lycopersicum var. Jingfan 101) begins to soften 9 days earlier than that transformed with a net vector. Fruit of AcoPG3-overexpressing pineapple (APG3-2) begins to soften 6 days earlier than that transformed with a net vector. Fruit of MPG3-1, a pineapple line in which AcoPG3 is mutated, begins to soften 31 days later than that transformed with a net vector. The sequence of polygalacturonase activities in fruit from the highest to the lowest was APG3-2, wild type, MPG3-1. The same sequences were also found in the liquid content of apoplast and the electrolyte leakage of pineapple pulp. The order of methyl-esterified pectin content in the pulp cell wall, from the highest to the lowest, was MPG3-1, wild type, and APG3-2. The same order was also observed for the contents of non-methyl-esterified homogalacturonan and rhamnogalacturonan-I in the pulp cell wall. The AcoPG3 mutation resulted in a decrease in polygalacturonase activity in pineapple fruit, decreasing the degradation of methyl-esterified pectin, non-methyl-esterified homogalacturonan, galactan and rhamnogalacturonan-I in the pulp cell wall. Fruit softening can be deferred, and the shelf life can be extended by mutating the AcoPG3 gene.

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