A larval expressed chemosensory protein involved in recognition of anthocyanins in Spodoptera litura (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Dong, Xiaolin

作者： Dong, Xiaolin;Huang, Yaling;Pei, Yanfang;Chen, Ling;Tan, Tianliang;Li, Chuanren;Huang, Yaling;Fu, Lijun;Xiang, Fayun

作者机构：

关键词： gustatory; chemosensory protein; feeding behavior; anthocyanin; Spodoptera litura

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.4； 五年影响因子：2.4 ）

ISSN： 0022-0493

年卷期： 2024 年 117 卷 6 期

页码：

收录情况： SCI

摘要： Anthocyanins are secondary metabolites which act as diverse functions during plant growth. Insects can discriminate host plants by their sensitive gustatory systems. It is hypothetical that chemosensory proteins (CSPs) play a crucial role in regulating this behavioral process. However, the underlying molecular mechanisms remain obscure. In the present study, we characterized a CSP SlitCSP8 from the Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Quantitative real-time-polymerase chain reaction analysis demonstrated that SlitCSP8 was mainly expressed in the head of the 7th S. litura larvae, especially labrum. Further, recombinant SlitCSP8 was obtained using bacterial expression system. Fluorescence competitive binding assays demonstrated that the purified SlitCSP8 exhibited a strong binding affinity to anthocyanins, a natural compound derived from the host plant. Silencing SlitCSP8through RNAi significantly reduced the sensitivity of S. litura larvae to anthocyanins-treated leaf disks, the development from larva to pupae was not affected. These data provide insight into the molecular basis that CSP8 can detect anthocyanins in host plants by chemosensory system of insects. It can be further used in designing novel optimal food attractant targeting to the CSPs for pest control.

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