Molecular cloning of cryptochrome 1 from Lilium x formolongi and the characterization of its photoperiodic flowering function in Arabidopsis

文献类型： 外文期刊

第一作者： Xiao-Mei Wu

作者： Xiao-Mei Wu;Xiao-Ru Wei;Li, Ze;Ji-Ren Chen;Hai-Xia Chen;Fu-Xiang Cao;Yu-Fan Li;Gui-Xia Jia;Gui-Xia Jia;Si-Xiang Zheng;Jian-Hong Li

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关键词： Cryptochrome; Lilium x formolongi; Flower induction; Photoperiod; Function analysis

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 316 卷

页码：

收录情况： SCI

摘要： Lilium x formolongi is an important cut flower species that is able to flower within a year following seed propagation, with flower induction that is very sensitive to the photoperiod. Cryptochmmes are blue/UV-A light receptors that regulate many important plant growth and development processes, including photoperiodic flowering. In this study, we isolated the cryptochrome 1 (CRY1) gene from L. x formolongi and analyzed its function in transgenic Arabidopsis. The predicted LfCRY1 protein was strongly homologous to other CRY1 proteins. The transcription of LfCRY1 was induced by blue light, with LfCRY1 exhibiting its highest expression and diurnal expression patterns during the flowering-induction stage under both long-day (LD) and short-day (SD) photoperiods. Overexpression of LfCRY1 in Arabidopsis promoted flowering under LDs but not SDs and inhibited hypocotyl elongation under blue light. The LfCRY1 protein was located in both the nucleus and cytoplasm. LfCRY1 interacted with the important flowering activator LfCOL9 in both yeast and onion cells. These results provide functional evidence for the role of LfCRY1 in controlling photoperiodic flowering under LDs and indicate that LfCRY1 may be a counterpart of AtCRY1. Understanding the role of LfCRY1 in photoperiodic flowering is beneficial for the molecular breeding of lilies with shorter vegetative stages.

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