Isolation, Identification, Biological Characterization, and Pathogenicity of Entomopathogenic Fungus from the Larvae of the Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae)

文献类型： 外文期刊

第一作者： Ma, Youhua

作者： Ma, Youhua;Qin, Minggang;Zeng, Yuanfang;Shen, Yinyin;Lai, Youpeng;Lu, Guangxin

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关键词： Evergestis extimalis; entomopathogenic fungi; Mucor hiemalis; cultivation conditions; pathogenicity

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

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年卷期： 2025 年 14 卷 5 期

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收录情况： SCI

摘要： At Qinghai agriculture districts, Evergestis extimalis poses a significant threat to spring rapeseed cultivation through its larvae burring into the rapeseed kernels and feeding seeds. To protect the ecological environment of the Qinghai-Tibet Plateau, it is essential to research and develop biological control technologies for pest management. In this study, we isolated and purified a new entomopathogenic fungus from the carcasses of E. extimalis larvae, which was identified as Mucor hiemalis based on morphological characteristics combined with ITS rDNA and 18S rDNA sequence analyses. Subsequently, the optimal growth conditions for the strain were determined as follows: SDAY medium, fructose as the carbon source, peptone as the nitrogen source, 25 degrees C, pH 6.0-7.0, and a 0:24 (light:dark) photoperiod.However, UV can significantly reduce fungal spore production. The bioassay result shows its pathogenicity was a concentration-dependent effect on E. extimalis, and younger larvae were more susceptible. With 1 x 108 spores/mL inoculated, survival of second instar larvae decreased by the greenhouse pot experiment. In conclusion, M. hiemalis exhibits a significant biocontrol potential against E. extimalis.

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