Investigation of heat stress responses and adaptation mechanisms by integrative metabolome and transcriptome analysis in tea plants (Camellia sinensis)

文献类型： 外文期刊

第一作者： Huang, Feiyi

作者： Huang, Feiyi;Lei, Yu;Duan, Jihua;Kang, Yankai;Luo, Yi;Ding, Ding;Chen, Yingyu;Li, Saijun

作者机构：

关键词： Camellia sinensis; Heat stress; Molecular chaperone; Flavonoids; Photosynthesis

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.6； 五年影响因子：4.9 ）

ISSN： 2045-2322

年卷期： 2024 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Extreme high temperature has deleterious impact on the yield and quality of tea production, which has aroused the attention of growers and breeders. However, the mechanisms by which tea plant varieties respond to extreme environmental heat is not clear. In this study, we analyzed physiological indices, metabolites and transcriptome differences in three different heat-tolerant tea plant F1 hybrid progenies. Results showed that the antioxidant enzyme activity, proline, and malondialdehyde were significantly decreased in heat-sensitive 'FWS' variety, and the accumulation of reactive oxygen molecules such as H2O2 and O-2(-) was remarkably increased during heat stress. Metabolomic analysis was used to investigate the metabolite accumulation pattern of different varieties in response to heat stress. The result showed that a total of 810 metabolites were identified and more than 300 metabolites were differentially accumulated. Transcriptional profiling of three tea varieties found that such genes encoding proteins with chaperon domains were preferentially expressed in heat-tolerant varieties under heat stress, including universal stress protein (USP32, USP-like), chaperonin-like protein 2 (CLP2), small heat shock protein (HSP18.1), and late embryogenesis abundant protein (LEA5). Combining metabolomic with transcriptomic analyses discovered that the flavonoids biosynthesis pathway was affected by heat stress and most flavonols were up-regulated in heat-tolerant varieties, which owe to the preferential expression of key FLS genes controlling flavonol biosynthesis. Take together, molecular chaperons, or chaperon-like proteins, flavonols accumulation collaboratively contributed to the heat stress adaptation in tea plant. The present study elucidated the differences in metabolite accumulation and gene expression patterns among three different heat-tolerant tea varieties under extreme ambient high temperatures, which helps to reveal the regulatory mechanisms of tea plant adaptation to heat stress, and provides a reference for the breeding of heat-tolerant tea plant varieties.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Aneurinibacillus uraniidurans sp. nov., a uranium- resistant bacterium isolated from uranium- contaminated soil

    作者：Zhang, Lijuan;Zhang, Lijuan;Wang, Ning;Huang, Wei;Wang, Wei;Luo, Yi;Wu, Longyuan;Wang, Suling;Sheng, Jiandong;Song, Bo;Zheng, Guofeng;Liu, Zaidao;Zhang, Yongwen

    关键词：Aneurinibacillus; uranium tolerance

  • Segmentation of wheat scab fungus spores based on CRF_ResUNet plus

    作者：Zhang, Dongyan;Zhang, Wenhao;Cheng, Tao;Lei, Yu;Gu, Chunyan;Zhang, Dongyan;Zhang, Wenhao;Cheng, Tao;Lei, Yu;Qiao, Hongbo;Guo, Wei;Yang, Xue;Gu, Chunyan

    关键词：Spore segmentation; UNet plus plus; Residual blocks; Fully connected conditional random fields; Wheat scab

  • miR828a-CsMYB114 Module Negatively Regulates the Biosynthesis of Theobromine in Camellia sinensis

    作者：Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Xie, Siyi;Shang, Fanghuizi;Wen, Shuai;Wu, Ting;Jin, Huiying;Liu, Guizhi;Hu, Jinyu;Su, Qin;Huang, Mengdi;Zhu, Qian;Zhou, Biao;Zhu, Lihua;Peng, Lvwen;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Xie, Siyi;Shang, Fanghuizi;Wen, Shuai;Wu, Ting;Jin, Huiying;Liu, Guizhi;Hu, Jinyu;Su, Qin;Huang, Mengdi;Zhu, Qian;Zhou, Biao;Zhu, Lihua;Peng, Lvwen;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Xie, Siyi;Shang, Fanghuizi;Wen, Shuai;Wu, Ting;Jin, Huiying;Liu, Guizhi;Hu, Jinyu;Su, Qin;Huang, Mengdi;Zhu, Qian;Zhou, Biao;Zhu, Lihua;Peng, Lvwen;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Sandhu, Devinder;Huang, Feiyi

    关键词："Jianghua Kucha"; theobromine; broadly targeted metabolomic analysis; gene suppression; tea plant

  • Compendium of 5810 genomes of sheep and goat gut microbiomes provides new insights into the glycan and mucin utilization

    作者：Zhang, Ke;Zhang, Ting;Xu, Yangbin;Lei, Yu;Liu, Gongwei;Qian, Quan;Mao, Yunrui;Kalds, Peter;Yang, Yuxin;Wang, Xiaolong;Chen, Yulin;He, Chong;Wang, Meili;Wang, Lei;Suo, Langda;Wu, Yujiang;Cuoji, Awang;Suo, Langda;Wu, Yujiang;Cuoji, Awang;Guo, Mengmeng;Guo, Jiazhong;Brugger, Daniel;Gan, Shangquan;Wang, Xiaolong;Chen, Yulin;Wang, Xiaolong;Chen, Yulin;Zhao, Fangqing

    关键词：

  • Soil Microbial Community Characteristics and Their Effect on Tea Quality under Different Fertilization Treatments in Two Tea Plantations

    作者：Lei, Yu;Ding, Ding;Duan, Jihua;Luo, Yi;Huang, Feiyi;Kang, Yankai;Chen, Yingyu;Li, Saijun;Lei, Yu;Ding, Ding;Duan, Jihua;Luo, Yi;Huang, Feiyi;Kang, Yankai;Chen, Yingyu;Li, Saijun;Lei, Yu;Ding, Ding;Duan, Jihua;Luo, Yi;Huang, Feiyi;Kang, Yankai;Chen, Yingyu;Li, Saijun

    关键词：metagenomics; amplicon sequencing; soil microbe; organic fertilizer; soil properties

  • Effect of Leaf Grade on Taste and Aroma of Shaken Hunan Black Tea

    作者：Wang, Kuofei;Xie, Nianci;Xu, Hao;Liu, Changwei;Huang, Jianan;Liu, Zhonghua;Wang, Kuofei;Xie, Nianci;Xu, Hao;Liu, Changwei;Huang, Jianan;Liu, Zhonghua;Xiao, Yangbo;Xie, Nianci;Li, Saijun;Zhang, Shuguang;Yin, Xia;Huang, Jianan;Liu, Zhonghua;Huang, Jianan;Liu, Zhonghua

    关键词：shaken Hunan black tea; leaves grade; taste; aroma; HS-SPME/GC-MS

  • mRNA-miRNA analyses reveal the involvement of CsbHLH1 and miR1446a in the regulation of caffeine biosynthesis in Camellia sinensis

    作者：Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Shang, Fanghuizi;Xie, Siyi;Chen, Zhenyan;Zhang, Xiangqin;Hu, Jinyu;Liu, Guizhi;Su, Qin;Huang, Mengdi;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Shang, Fanghuizi;Xie, Siyi;Chen, Zhenyan;Zhang, Xiangqin;Hu, Jinyu;Liu, Guizhi;Su, Qin;Huang, Mengdi;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Jin, Qifang;Wang, Zhong;Chen, Lan;Shao, Chenyu;Shang, Fanghuizi;Xie, Siyi;Hu, Jinyu;Liu, Guizhi;Su, Qin;Huang, Mengdi;Liu, Zhonghua;Huang, Jianan;Tian, Na;Liu, Shuoqian;Sandhu, Devinder;Huang, Feiyi

    关键词：

微信小程序