Reusable molecularly imprinted electrochemiluminescence assay for kanamycin based on ordered mesoporous carbon loaded with indium oxide nanoparticles and carbon quantum dots

文献类型: 外文期刊

第一作者: Li, Shuhuai

作者: Li, Shuhuai;Wu, Yuwei;Pang, Chaohai;Ma, Xionghui;Wang, Mingyue;Luo, Jinhui;Zhi, Xu;Li, Shuhuai;Wu, Yuwei;Pang, Chaohai;Ma, Xionghui;Wang, Mingyue;Luo, Jinhui;Zhi, Xu;Li, Bei

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关键词: Ordered mesoporous carbon; Indium oxide; Carbon quantum dots; Molecular imprinted sensor; Kanamycin

期刊名称:MICROCHIMICA ACTA ( 影响因子:6.408; 五年影响因子:5.888 )

ISSN: 0026-3672

年卷期: 2022 年 189 卷 11 期

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收录情况: SCI

摘要: A highly sensitive kanamycin electrochemiluminescence (ECL) switch sensor was constructed. A signal element consisting of ordered mesoporous carbon loaded with indium oxide nanoparticles/carbon quantum dots (OMC/In2O3/C-dots) was assembled on the surface of a gold electrode. Then, a molecularly imprinted polymer (MIP) was prepared on the modified electrode surface using kanamycin as the template molecule and o-aminophenol as the functional monomer. After kanamycin elution, the prepared sensor retained specific kanamycin recognition sites. OMC/In2O3 effectively amplified the ECL signal of the C-dots, thereby enhancing the detection sensitivity, whereas kanamycin quenched the signal. Therefore, the imprinted sites acted as a switch, providing a new method for detecting kanamycin. Under the optimal experimental conditions, the concentration of kanamycin was proportional to the degree of ECL quenching within a linear range of 5-4500 x 10(-12) mol L-1 at 0.8 V (vs. Ag/AgCl electrode electrode), and the detection limit was 5.8 x 10(-13) mol L-1. When applied to the detection of kanamycin in actual samples, such as chicken, duck, pork, and milk, the recovery for spiked samples was in the range 92.7-110%.

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