Fumigation practice combined with organic fertilizer increase antibiotic resistance in watermelon rhizosphere soil

文献类型： 外文期刊

第一作者： Du, Shuai

作者： Du, Shuai;Ge, An-Hui;Zhang, Li-Mei;Ge, An-Hui;Zhang, Li-Mei;Shen, Ju-Pei;Liang, Zhi-Huai;Xiang, Ji-Fang;Xiao, Ji-Ling;Zhang, Yi;Du, Shuai;Liu, Yu-Rong;Shen, Ju-Pei

作者机构：

关键词： Antibiotic resistance gene; Facility agriculture; Mobile genetic element; Organic fertilizer; Rhizosphere soil; Soil fumigant

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2022 年 805 卷

页码：

收录情况： SCI

摘要： Chemical fumigants and organic fertilizer are commonly used in facility agriculture to control soil-borne diseases and promote soil health. However, there is a lack of evidence for the effect of non-antibiotic fumigants on the distribution of antibiotic resistance genes (ARGs) in plant rhizosphere soils. Here, the response of a wide spectrum of ARGs and mobile genetic elements (MGEs) to dazomet fumigation practice in the rhizosphere soil of watermelon was investigated along its branching, flowering and fruiting growth stages in plastic shelters using high throughput quantitative PCR approach. Our results indicated that soil fumigation combined with organic fertilizer application significantly increased the relative abundance of ARGs and MGEs in the rhizosphere soil of watermelon plant. The positive correlations between the relative abundance of ARGs and MGEs suggested that soil fumigation might increase the horizontal gene transfer (HGT) potential of ARGs. This result was further confirmed by the enhanced associations between ARG and MGE subtypes in the networks of fumigation treatments. Moreover, bipartite associations between ARGs/MGEs and microbial communities (bacteria and fungi) revealed a higher percentage of linkage between MGEs and microbial taxa in the fumigated soils. Structural equation model analysis further suggested that the increases in antibiotic resistance after fumigation and organic fertilizer application were mainly driven by MGEs and fungal community. Together, our results provide vital evidence that dazomet fumigation process combined with organic fertilizer in plastic shelters has the great potential to promote ARGs' dissemination in the rhizosphere, and raise cautions of the acquired resistance by soil-borne fungal pathogen and the potential spreading of ARGs along soil-plant continuum. (c) 2021 Elsevier B.V. All rights reserved.

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