Homolog of Pea SGR Controls Stay-Green in Faba Bean (Vicia faba L.)

文献类型： 外文期刊

第一作者： Chen, Jingbin

作者： Chen, Jingbin;Zhou, Huimin;Yuan, Xingxing;He, Yaming;Yan, Qiang;Lin, Yun;Wu, Ranran;Liu, Jinyang;Xue, Chenchen;Chen, Xin;Chen, Jingbin;Yuan, Xingxing;Yan, Qiang;Lin, Yun;Wu, Ranran;Liu, Jinyang;Xue, Chenchen;Chen, Xin;Zhou, Huimin;He, Yaming

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关键词： faba bean; stay-green; green cotyledon; SGR; chlorophyll degradation

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 5 期

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收录情况： SCI

摘要： Faba bean is an important legume crop consumed as a vegetable or snack food, and its green cotyledons could present an attractive color for consumers. A mutation in SGR causes stay-green in plants. In this study, vfsgr was identified from a green-cotyledon-mutant faba bean, SNB7, by homologous blast between the SGR of pea and the transcriptome of faba bean. Sequence analysis revealed that a SNP at position 513 of the CDS of VfSGR caused a pre-stop codon, resulting in a shorter protein in the green-cotyledon faba bean SNB7. A dCaps marker was developed according to the SNP that caused the pre-stop, and this marker was completely associated with the color of the cotyledon of faba bean. SNB7 stayed green during dark treatment, while the expression level of VfSGR increased during dark-induced senescence in the yellow-cotyledon faba bean HST. Transient expression of VfSGR in Nicotiana. benthamiana leaves resulted in chlorophyll degradation. These results indicate that vfsgr is the gene responsible for the stay-green of faba bean, and the dCaps marker developed in this study provides a molecular tool for the breeding of green-cotyledon faba beans.

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