The chromosome-level Stevia genome provides insights into steviol glycoside biosynthesis

文献类型： 外文期刊

第一作者： Xu, Xiaoyang

作者： Xu, Xiaoyang;Yuan, Haiyan;Huang, Suzhen;Sun, Yuming;Zhang, Ting;Liu, Qingquan;Tong, Haiying;Zhang, Yongxia;Wang, Yinjie;Hou, Menglan;Yang, Yongheng;Xu, Xiaoyang;Yuan, Haiyan;Huang, Suzhen;Sun, Yuming;Zhang, Ting;Liu, Qingquan;Tong, Haiying;Zhang, Yongxia;Wang, Yinjie;Hou, Menglan;Yang, Yongheng;Yu, Xiaqing;Liu, Chunxiao;Wu, Lei

作者机构：

期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）

ISSN： 2662-6810

年卷期： 2021 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Stevia (Stevia rebaudiana Bertoni) is well known for its very sweet steviol glycosides (SGs) consisting of a common tetracyclic diterpenoid steviol backbone and a variable glycone. Steviol glycosides are 150-300 times sweeter than sucrose and are used as natural zero-calorie sweeteners. However, the most promising compounds are biosynthesized in small amounts. Based on Illumina, PacBio, and Hi-C sequencing, we constructed a chromosome-level assembly of Stevia covering 1416 Mb with a contig N50 value of 616.85 kb and a scaffold N50 value of 106.55 Mb. More than four-fifths of the Stevia genome consisted of repetitive elements. We annotated 44,143 high-confidence protein-coding genes in the high-quality genome. Genome evolution analysis suggested that Stevia and sunflower diverged similar to 29.4 million years ago (Mya), shortly after the whole-genome duplication (WGD) event (WGD-2, similar to 32.1 Mya) that occurred in their common ancestor. Comparative genomic analysis revealed that the expanded genes in Stevia were mainly enriched for biosynthesis of specialized metabolites, especially biosynthesis of terpenoid backbones, and for further oxidation and glycosylation of these compounds. We further identified all candidate genes involved in SG biosynthesis. Collectively, our current findings on the Stevia reference genome will be very helpful for dissecting the evolutionary history of Stevia and for discovering novel genes contributing to SG biosynthesis and other important agronomic traits in future breeding programs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序