Improving the Thermo-Activity and -Stability of Pectate Lyase from Dickeya dadantii DCE-01 for Ramie Degumming

文献类型： 外文期刊

第一作者： Xu, Huan

作者： Xu, Huan;Duan, Shengwen;Feng, Xiangyuan;Yang, Qi;Zheng, Ke;Peng, Yuande;Cheng, Lifeng

作者机构：

关键词： Dickeya dadantii DCE-01; pectate lyase; site-directed mutagenesis; thermal stability; degumming

期刊名称：PROCESSES （ 影响因子：3.352； 五年影响因子：3.338 ）

ISSN：

年卷期： 2021 年 9 卷 12 期

页码：

收录情况： SCI

摘要： To improve the thermal stability of pectate lyase for ramie degumming, we modified the novel pectate lyase gene (pelG403) derived from the Dickeya dadantii DCE-01 high-efficiency ramie degumming strain by site-directed mutagenesis. Twelve mutants were acquired, wherein a prospective mutant (A129V) showed better enzyme activity and thermal stability. Compared with the wild type (PelG403), the specific enzyme activity and the optimal reaction temperature of A129V in the fermentation broth increased by 20.1%, and 5 degrees C, respectively. Under the conditions of 55 degrees C and pH 9.0, the weightlessness rate of ramie raw materials of A129V increased by 6.26%. Therefore, this study successfully improved the enzyme activity and heat resistance of PelG403 in an alkaline environment, which may contribute to the development of enzyme preparations and the elucidation of the mechanism for ramie bio-degumming.

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