The IolR regulon contributes to Aeromonas hydrophila virulence in channel catfish (Ictalurus punctatus)

文献类型： 外文期刊

第一作者： Xu, Tingbi

作者： Xu, Tingbi;Hossain, Mohammad J.;Waits, Damien S.;Thurlow, Charles M.;Liles, Mark R.;Dong, Yuhao;Liu, Yongjie;Srikumar, Shabarinath;Terhune, Jeffery S.;Sun, Dawei;Newton, Joseph C.;Sun, Dawei

作者机构：

关键词： Aeromonas hydrophila (vAh); IolR regulon; Transcriptome analysis; Catfish; Fish challenge

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2026 年 610 卷

页码：

收录情况： SCI

摘要： Hypervirulent Aeromonas hydrophila (vAh) causes devastating damage in aquaculture worldwide. All vAh strains utilize myo-inositol (MI) as a sole carbon source prompted the previous study of the MI pathway in virulence. A range of gene-deletion mutants in the MI pathway were investigated through in vivo fish challenges to identify the insignificant role of MI pathway in virulence. However, the unexpected virulence of iolR mutants suggested the importance of IolR regulon as the transcriptional regulator for virulence. This study discerned the role of the IolR regulons on the pathogenesis of vAh. The A. hydrophila ML09-119 iolR gene was mutated to obtain different iolR expression levels. The results showed that increasing IolR expression caused decreased mortality in channel catfish 6 h post challenge, suggesting the role of IolR in repressing virulence gene transcription. A transcriptome analysis was conducted for ML09-119 and mutants. The results indicated that multiple putative genes involved in MI metabolism, fimbrial biogenesis, cell envelope integrity, and horizontal gene transfer might have IolRregulated transcription, with a palindromic sequence identified as the putative IolR binding site. Notably, when comparing phenotypes of iolR mutants of the channel catfish isolate ML09-119 and the carp isolate NJ-35, the regulation of biofilm formation and auto-aggregation were only observed for the carp isolate, which may reflect host-specific transcriptional regulation. Further study is necessary to understand the differences in the IolR regulon for carp and catfish isolates, as well as the contribution of each of the IolR-regulated genes to vAh pathogenesis.

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