Co-incorporation of Chinese milk vetch (Astragalus sinicus L.) and chemical fertilizers alters microbial functional genes supporting short-time scale positive nitrogen priming effects in paddy soils

文献类型： 外文期刊

第一作者： Wang, Limin

作者： Wang, Limin;He, Chunmei;Huang, Dongfeng;Liu, Cailing;Li, Qinghua;Huang, Yibin;Li, Juan;Wang, Fei;Yu, Juhua

作者机构：

关键词： green manure; gross priming effect; incorporation practice; microbial mechanism; net priming effect; rice field soil

期刊名称：PEDOSPHERE （ 影响因子：5.2； 五年影响因子：5.3 ）

ISSN： 1002-0160

年卷期： 2024 年 34 卷 3 期

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收录情况： SCI

摘要： Nitrogen (N) priming is a microbially mediated biochemical process as affected by different incorporation practices. However, little information is known about the microbial mechanisms driving the response of N priming to co-operation of Chinese milk vetch (CMV, Astragalus sinicus L.) and different rates of chemical fertilizers in paddy soils in South China. Here, an anaerobic incubation experiment was conducted to study N priming effects (PE) and their relationships with soil microbial functional genes after CMV incorporation alone (M), co -incorporation of CMV with 100% (normal dosage) chemical fertilizers (MC100), and co -incorporation of CMV with 80% chemical fertilizers (MC80). Co -incorporation of CMV and chemical fertilizers enhanced the short -time scale (the first 20 d of incubation) positive PE of N, while no significant differences existed among the three treatments on day 60 or 90 of incubation ( P > 0.05). Compared with the M treatment, gross priming effect (GPE) in the MC100 and MC80 treatments significantly increased by 34.0% and 31.3%, respectively, and net priming effect (NPE) increased by 47.7% and 47.8%, respectively, during the first 20 d of incubation ( P < 0.05). This was likely attributed to soil nutrient availability and added substrate quality. The MC100 and MC80 treatments increased the gdhA gene abundance by 5.0% and 9.8%, increased the gdh2 gene abundance by 12.7% and 45.7%, and increased the nasB gene abundance by 9.5% and 41.4%, respectively, in comparison with the M treatment on day 20 of incubation. Correlation analyses indicated that soil microbial functional genes involved in N mineralization ( gdhA and gdh2 ), assimilatory nitrate reduction ( nasB ), and nitrification ( amoB ) were significantly correlated with N priming under different incorporation practices during the incubation period ( P < 0.05). Thus, co -incorporation of CMV and chemical fertilizers can regulate soil microbial community functional gene structure, which may accelerate mineralization and assimilatory nitrate reduction and inhibit nitrification, thereby increasing the short-term positive PE of N in the present study.

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