Inactivated LMBV vaccine with nano-aluminum - aluminum adjuvant provided immune protection in largemouth bass ( Micropterus Salmoides) )
文献类型： 外文期刊
第一作者： Luo, Xia
作者： Luo, Xia;Ma, Baofu;Chang, Ouqin;Fu, Xiaozhe;Liang, Hongru;Niu, Yinjie;Lin, Qiang;Liu, Lihui;Li, Ningqiu;Wang, Ruoxuan;Lv, Aijun
作者机构：
关键词： Largemouth bass; LMBV; Inactivated vaccine; Nano-aluminum adjuvant; Potentiation effects
期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）
ISSN： 0044-8486
年卷期： 2025 年 595 卷
页码：
收录情况： SCI
摘要： Largemouth bass ranavirus (LMBV) has caused severe economic losses in largemouth bass (Micropterus salmoides) aquaculture. Vaccination is acknowledged as an efficient strategy to prevent viral diseases in fish. In this study, an inactivated virus vaccine with nano-aluminum adjuvant was successfully developed to protect largemouth bass effectively against LMBV. LMBV was inactivated by formalin and mixed with nano-aluminum adjuvant under moderate agitation. The nano-aluminum hydroxide adjuvant showed a cuboidal structure under the scanning transmission electron microscope (STEM), scanning electron microscope (SEM), and transmission electron microscope (TEM), and LMBV antigen-adsorbing rate was 39.49 % superior to those of micro-aluminum hydroxide and IMS1312 adjuvants. The results from both external and internal damage assessments indicated that the nano-aluminum vaccine had good safety for Micropterus salmoides. The relative percentage of survival was 80 % with a booster immunization in largemouth bass. The expression levels of immune-related genes such as TNF-alpha, IFN, and IL-8 were significantly up-regulated in the spleen of fish immunized by nano-aluminum adjuvant vaccine compared to those in inactivated LMBV and control groups. The neutralizing antibody in nano-aluminum adjuvant vaccine group showed higher abundance and lasted for a longer period. Lymphocytes from the head kidney of largemouth bass in the nano-aluminum adjuvant vaccine group showed stronger proliferation activity in vitro post-stimulation with ConA. Moreover, stronger migration of the dendritic cells was induced by the nano-aluminum adjuvant vaccine. Collectively, our findings suggested that the inactivated LMBV vaccine with nano-aluminum adjuvant could provide efficient protection for largemouth bass against LMBV infection. The nano-aluminum adjuvant has potential application with inactivated viral vaccines in fish.
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