Epidemiological Survey and Risk Factor Analysis of 14 Potential Pathogens in Golden Snub-Nosed Monkeys at Shennongjia National Nature Reserve, China

文献类型： 外文期刊

第一作者： Qi, Mingpu

作者： Qi, Mingpu;Wang, Qiankun;Wang, Yu;Chen, Yingyu;Hu, Changmin;Dawood, Ali Sobhy;Zubair, Muhammad;Chen, Jianguo;Robertson, Ian Duncan;Chen, Huanchun;Guo, Aizhen;Wang, Yu;Chen, Yingyu;Robertson, Ian Duncan;Chen, Huanchun;Guo, Aizhen;Chen, Yingyu;Robertson, Ian Duncan;Guo, Aizhen;Chen, Yingyu;Robertson, Ian Duncan;Guo, Aizhen;Yang, Wanji;Wu, Feng;Huang, Tianpeng;Dawood, Ali Sobhy;Zubair, Muhammad;Li, Xiang;Robertson, Ian Duncan

作者机构：

关键词： golden snub-nosed monkey; epidemiology; tuberculosis; adenovirus; macacine herpesvirus 1; cytomegalovirus; simian foamy virus; hepatitis A virus

期刊名称：PATHOGENS （ 影响因子：3.7； 五年影响因子：3.7 ）

ISSN：

年卷期： 2023 年 12 卷 3 期

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收录情况： SCI

摘要： Golden snub-nosed monkeys (Rhinopithecus roxellanae) belong to Class A, the highest level of endangered primate species. Exploring the infection status of potential pathogens in golden snub-nosed monkeys is important for controlling associated diseases and protecting this species. The objective of this study was to investigate the seroprevalence for a number of potential pathogens and the prevalence of fecal adenovirus and rotavirus. A total of 283 fecal samples were collected from 100 golden snub-nosed monkeys in December 2014, June 2015, and January 2016; 26 blood samples were collected from 26 monkeys in June 2014, June 2015, January 2016 and November 2016 at Shennongjia National Reserve in Hubei, China. The infection of 11 potential viral diseases was examined serologically using an Indirect Enzyme-linked Immunosorbent Assay (iELISA) and Dot Immunobinding Assays (DIA), while the whole blood IFN-gamma in vitro release assay was used to test tuberculosis (TB). In addition, fecal Adenovirus and Rotavirus were detected using Polymerase Chain Reaction (PCR). As a result, the Macacine herpesvirus-1 (MaHV-1), Golden snub-nosed monkey cytomegalovirus (GsmCMV), Simian foamy virus (SFV) and Hepatitis A virus (HAV) were detected with the seroprevalence of 57.7% (95% CI: 36.9, 76.6), 38.5% (95% CI: 20.2, 59.4), 26.9% (95% CI: 11.6, 47.8), and 7.7% (95% CI: 0.0, 84.2), respectively. Two fecal samples tested positive for Adenovirus (ADV) by PCR, with a prevalence of 0.7% (95% CI: 0.2, 2.5), and further, the amplification products were sequenced. Phylogenetic analysis revealed that they belonged to the HADV-G group. However, other pathogens, such as Coxsackievirus (CV), Measles virus (MeV), Rotavirus (RV), Simian immunodeficiency virus (SIV), Simian type D retroviruses (SRV), Simian-T-cell lymphotropic virus type 1 (STLV-1), Simian varicella virus (SVV), Simian virus 40 (SV40) and Mycobacterium tuberculosis complex (TB) were negative in all samples. In addition, a risk factor analysis indicated that the seroprevalence of MaHV-1 infection was significantly associated with old age (>= 4 years). These results have important implications for understanding the health status and conservation of the endangered golden snub-nosed monkey population at Shennongjia Nature Reserve.

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