Two electrochemiluminescence immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified crops

文献类型： 外文期刊

第一作者： Zeng, Haijuan

作者： Zeng, Haijuan;Zhang, Minghao;Liu, Hua;Wang, Jinbin;Zeng, Haijuan;Liu, Hua;Wang, Jinbin;Zeng, Haijuan;Zhang, Minghao;Liu, Juan;Zhu, Lemei;Feng, Dongsheng

作者机构：

关键词： Signal-reduced or -enhanced model; Electrochemiluminescence immunosensor; CdSe; ZnS QDs; Nanocomposites; CP4-EPSPS protein

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 428 卷

页码：

收录情况： SCI

摘要： Two different models of electrochemiluminescence (ECL) immunosensors for the sensitive and quantitative detection of the CP4-EPSPS protein in genetically modified (GM) crops were proposed in this study. One was a signal-reduced ECL immunosensor based on nitrogen-doped graphene, graphitic carbon nitride and polyamideamine (GN-PAMAM-g-C3N4) composites as the electrochemically active substance. The other model was a signalenhanced ECL immunosensor based on a GN-PAMAM modified electrode for the detection of CdSe/ZnS quantum dots (QDs)-labeled antigens. The ECL signal responses of the reduced and enhanced immunosensors linearly decreased as the increase of the soybean RRS and RRS-QDs content in the range of 0.05% to 1.5% and 0.025% to 1.0%, with the limits of detection of 0.03% and 0.01% (S/N = 3), respectively. Both of the ECL immunosensors showed good specificity, stability, accuracy, and reproducibility in the analysis of real samples. The results indicate that the two immunosensors provide an ultra-sensitive and quantitative approach for the determination of the CP4-EPSPS protein. Due to their outstanding performances, the two ECL immunosensors could be useful tools for achieving the effective regulation of GM crops.

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