A novel bis-reaction-triggered cascade fluorescent probe for improved specific detection and biological visualization of Cys over Hcy/GSH

文献类型： 外文期刊

第一作者： Cao, Chen

作者： Cao, Chen;Jing, Chunlin;Feng, Yan;Song, Xuerui;Liu, Weisheng;Zhang, Guolin;Dou, Wei;Cao, Chen;Jing, Chunlin;Feng, Yan;Song, Xuerui;Liu, Weisheng;Zhang, Guolin;Dou, Wei;Ru, Jiaxi;Ru, Jiaxi

作者机构：

关键词： Bis-reaction-triggered; Cascade-responsive; Specific detection of Cys; Two-stage response; Dynamic cell-imaging

期刊名称：DYES AND PIGMENTS （ 影响因子：5.122； 五年影响因子：4.36 ）

ISSN： 0143-7208

年卷期： 2022 年 197 卷

页码：

收录情况： SCI

摘要： The striking similarities in chemical structures and properties between cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) bring huge interferences to achieve specific detection of Cys, limiting the further physiological and pathological researches on Cys. Herein, a bis-reaction-triggered cascade-responsive highly specific Cys fluorescent probe CT-C was constructed, containing coumarin group as fluorophore, alpha,beta-unsaturated ethanoyl as a Michael-addition site and 2-thiophenecarbonyl moiety as a nucleophilic substitution site, which overcame the interference of Hcy and GSH by two successive reaction processes. CT-C presented a two-stage fluorescence response towards Cys, a fast and strong "turn-on" response in the first process and a lengthy "on-off" response in the subsequent second process, while CT-C only displayed a very slow and weak fluorescence enhancement towards Hcy, and a slight fluorescence change towards GSH. To our knowledge, CT-C was the first reported probe utilizing two-stage cascade response for specific single detection of Cys. Furthermore, the novel two-stage response mode was also useful for the specific fluorescence visualization of exogenous Cys in BHK-21 cells.

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