Histology and metabonomics reveal the toxic effects of kresoxim-methyl on adult zebrafish

文献类型： 外文期刊

第一作者： Fang, Nan

作者： Fang, Nan;Zhang, Changpeng;Hu, Haoze;Li, Yanjie;Wang, Xiangyun;Zhao, Xueping;Jiang, Jinhua;Hu, Haoze

作者机构： Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Minist Agr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China;Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315832, Zhejiang, Peoples R China

关键词： Kresoxim-methyl; Metabonomics; Environmental concentration; Zebrafish

期刊名称：CHEMOSPHERE （ 2022影响因子：8.8； 五年影响因子：8.3 ）

ISSN： 0045-6535

年卷期： 2022 年 309 卷

页码：

收录情况： SCI

摘要： Studies have shown that kresoxim-methyl (KM) and other strobilurin fungicides have toxic effects on aquatic organisms. However, the potential deleterious effects of kresoxim-methyl (KM) on adult zebrafish regarding the ecological risk of environmental concentration remain unclear. Here, the histology and untargeted metabonomics was used to investigate the adverse effect on female zebrafish after exposure to KM at environmental concentration, aquatic life benchmark and one-half LC50 of adult zebrafish. Results demonstrated KM affected zebrafish liver, ovary and intestine development, blurred the boundary between hepatocytes or caused hepatic vacuoles, increased the percentage of perinucleolar oocyte and cortical alveolus oocyte, decreased intestinal goblet cells and disturbed villus and wall integrity after 21 d exposure. Metabonomics showed different concentrations of KM simultaneously influenced the metabolites annotated to vitamin digestion and absorption, serotonergic synapse, retinol metabolism, ovarian steroidogenesis and arachidonic acid (AA) metabolism in zebrafish liver. Results showed the decreased triglyceride and cholesterol levels, as well as the metabolic alterations in amino acid, lipid, vitamin and retinol metabolism caused by KM, might disturb the energy supply for normal liver development and oocyte maturation. In addition, KM altered the transcription of Tdo2a, Tdo2b, Ido1, Cxcl8b, Cyp7a, Cyp11a, Cyp11b, Cyp17a, Cyp19a, Hsd3 beta, Hsd17 beta, Pla2, Ptgs2a and Ptgs2b, the level of TG, TC, MDA, IFN, IL6 and Ca2+, and the activity of CAT, SOD Ca2+-ATPase in zebrafish liver. Moreover, cytoscape analysis suggested the disturbed AA metabolism caused by KM, might interconnect multiple metabolic pathways to share implicated function in the regulation of oocyte maturation and immune response. Current study brought us closer to an incremental understanding of the toxic mechanism of KM on adult zebrafish, indicated there was crosstalk among different regulatory pathways to regulate the metabolic disorders and biologically hazardous effects induced by KM.

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