Kinetic characters and resistance to inhibition of crude and purified brain acetylcholinesterase of three freshwater fishes by organophosphates

文献类型： 外文期刊

第一作者： Li, SN

作者： Li, SN;Xie, XC;Zhu, GN;Tan, YJ

作者机构：

关键词： Fish;Acetylcholinesterase (ache);Purification;Kinetics;Malathion;In-vitro;Biomarker;Butyrylcholinesterase;Serum

期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Acetylcholinesterase (AChE) was purified from the brain of three fresh-water fishes, topmouth gudgeon (Pseudo rasbora parva), goldfish (Carassius auratus auratus) and rainbow trout (Oncorrhychus mykiss, formerly named Salmo gairdneri) by PEG2000/phosphate-salt two phases extraction, DEAE-Sephadex A-50 and Sephadex G-200 chromatography. Kinetic characters and resistance to inhibition of crude and purified enzymes by organophosphates were then studied. Although the crude enzyme from the trout displayed a different specific activity, kinetic curved V-max, and sensitivity to inhibition by oxidized malathion and triazopos compared with the two cyprinoids (i.e. topmouth gudgeon and goldfish), the purified enzymes of all the three species showed no significant difference in all aspects. The result suggested a negligible intrinsic difference of brain AChEs among the tested species.

分类号： R99

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