Isolation and Expression Analysis of Fructokinase Genes from Citrus

文献类型： 外文期刊

第一作者： Qin, QP

作者： Qin, QP;Zhang, SL;Chen, JW;Xie, M;Jin, YF;Chen, KS;Asghar, S

作者机构：

关键词： citrus;fructokinase;gene cloning;expression analysis;fructose accumulation

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fructokinase (FRK) is of primary importance in phosphorylation of fructose in plants. Two genomic DNA fragments encoding putative fructokinase gene were isolated from Citrus unshiu Marc, using PCR, which were named Cufrk1 and Cufrk2. A cDNA sequence same to the exons' sequences of Cufrk1 was amplified by RT-PCR and the full-length cDNA sequences of this gene was isolated using rapid amplification of cDNA ends (RACE) from mature fruit, named CuFRK1 (GenBank number: AY561840). Sequencing analysis showed that the amino acid sequences were 68% identical between Cufrk1 and Cufrk2. The full-length cDNA of CuFRK1 was 1 459 bp in length and contained a complete ORF from 168 to 1 220 bp, encoding 350 amino acids with a calculated molecular weight of 37.5 kD and a predicted pI of 5.03. The deduced amino acids of CuFRK1 possessed two sugar-binding domains, three ATP-binding domains and were 62% to 78% identical to the previously characterized fructokinase genes of other plants. Northern analysis showed that transcripts of CuFRK1 (Cufrk1) and Cufrk2 were detected at a high level in leaves, fruit at early developmental stages, but undetectable in peels and stems. Their expression patterns were distinct in petals and mature fruit. Enzymatic analysis showed that the activity of citrus fructokinase was decreasing with the fruit development, coincided with the accumulation of fructose in fruit. There was a significantly negative correlation between the decreasing fructokinase activity and the increasing fructose content during fruit development.

分类号： Q94

  • 相关文献

[1]Cloning and differential expression analysis of defensin gene Cldef2.2 from watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Zhang, M.,Yang, X. P.,Xu, J. H.,Liu, G.,Yao, X. F.,Li, P. F.,Zhu, L. L..

[2]Cloning and expression analysis of four DELLA genes in peanut. An, J.,Zhao, Y. X.,Wang, X. J.,An, J.,Hou, L.,Li, C.,Wang, C. X.,Xia, H.,Zhao, C. Z.,Li, C. S.,Wang, X. J.,Li, C.,Zheng, Y. X..

[3]Isolation and expression features of hexose kinase genes under various abiotic stresses in the tea plant (Camellia sinensis). Li, Na-na,Qian, Wen-jun,Wang, Lu,Cao, Hong-li,Hao, Xin-yuan,Yang, Ya-jun,Wang, Xin-chao,Li, Na-na,Wang, Lu,Cao, Hong-li,Hao, Xin-yuan,Yang, Ya-jun,Wang, Xin-chao,Qian, Wen-jun.

[4]Identification, Expression, and Functional Analysis of the Fructokinase Gene Family in Cassava. Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Wang, Yun-Lin,Chen, Xia,Shang, Lu,Lu, Xiao-Hua,Li, Zhan,Hu, Xin-Wen,Wu, Xiao-Hui,Sun, Chong. 2017

[5]Cloning of Fructokinase Gene from Longan Fruit and Bioinformatics Analysis. Shuai, L.,Li, J.,Wu, Z. X.,Han, D. M.. 2014

[6]Expressing a Citrus ortholog of Arabidopsis ERF1 enhanced cold-tolerance in tobacco. Ma, Yanyan,Zhang, Jun,Chen, Jiao,Wu, Tianli,Zhu, Shiping,Yan, Shutang,Zhao, Xiaochun,Ma, Yanyan,Wu, Tianli,Zhong, Guangyan,Ma, Yanyan,Wu, Tianli,Zhong, Guangyan,Zhang, Lingyun.

[7]Dissipation, residues, and risk assessment of spirodiclofen in citrus. Sun, Haibin,Wang, Siwei,Liu, Yanping,Liu, Mingjin,Liu, Congyun.

[8]Arbuscular mycorrhizas improve plant growth and soil structure in trifoliate orange under salt stress. Zhang, Yi-Can,Zou, Ying-Ning,Bao, Qian,Wu, Qiang-Sheng,Zhang, Yi-Can,Zou, Ying-Ning,Wu, Qiang-Sheng,Wang, Peng,Wu, Qing-Hua,Wu, Qiang-Sheng.

[9]Inoculation response of mycorrhizas on morphology and physiological behaviour of trifoliate orange (Poncirus trifoliata) roots under salt stress. Wang, Peng,Srivastava, A. K.,Zhang, Yi-Can,Wu, Qiang-Sheng,Srivastava, A. K.,Wu, Qiang-Sheng.

[10]Comparative transcriptional profiling of orange fruit in response to the biocontrol yeast Kloeckera apiculata and its active compounds. Liu, Pu,Li, Guofeng,Liu, Pu,Chen, Kai,Long, Chao-an,Yang, Xiaoping. 2016

[11]Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Ding, Fang,Jin, Shuangxia,Hong, Ni,Wang, Guoping,Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Cao, Qing,Hong, Ni,Wang, Guoping. 2008

[12]Infection of wampee and lemon by the Citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) in China. Ding, F,Wang, G,Yi, G,Zhong, Y,Zeng, J,Zhou, B. 2005

[13]The Mining of Citrus EST-SNP and Its Application in Cultivar Discrimination. Jiang Dong,Ye Qing-liang,Wang Fu-sheng,Cao Li. 2010

[14]Genome-Wide Identification, Cloning and Functional Analysis of the Zinc/Iron-Regulated Transporter-Like Protein (ZIP) Gene Family in Trifoliate Orange (Poncirus trifoliata L. Raf.). Fu, Xing-Zheng,Zhou, Xue,Xing, Fei,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi,Fu, Xing-Zheng,Zhou, Xue,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi,Aarts, Mark G. M.. 2017

[15]Identification of Genes Associated with Lemon Floral Transition and Flower Development during Floral Inductive Water Deficits: A Hypothetical Model. Li, Jin-Xue,Li, Jin-Xue,Huang, Hua-Bin,Yue, Jian-Qiang,Gao, Jun-Yan,Du, Yu-Xia,Hou, Xiao-Jin,Zhu, Jiao,Hu, Cheng-Xiao,Hu, Chun-Gen,Zhang, Jin-Zhi,Zhou, Jing-Jing. 2017

[16]Physiological Effects and Fluorescence Labeling of Magnetic Iron Oxide Nanoparticles on Citrus (Citrus reticulata) Seedlings. Li, Junli,Hu, Jing,Xiao, Lian,Gan, Qiuliang,Li, Junli,Wang, Yunqiang. 2017

[17]Behaviour of spirotetramat residues and its four metabolites in citrus marmalade during home processing. Liu, Yanyu,Chen, Weijun,Sun, Dali,Gong, Lei,Jiang, Liyan,Jiao, Bining,Liu, Yanyu,Chen, Weijun,Sun, Dali,Gong, Lei,Jiang, Liyan,Jiao, Bining,Su, Xuesu,Jian, Qiu,Chen, Weijun,Jiao, Bining,Jiao, Bining.

[18]A deep-coverage BAC library for Ponkan mandarin and its further use in screening ethylene regulated genes. Wang, Miao,Tan, AnQun,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhong, GuangYan,Zhou, ZhiQin,Zhong, GuangYan,Wang, Miao,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhou, ZhiQin. 2010

[19]Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun. 2013

[20]Mycorrhizal symbiosis enhances tolerance to NaCl stress through selective absorption but not selective transport of K+ over Na+ in trifoliate orange. Wu, Qiang-Sheng,Zou, Ying-Ning,He, Xin-Hua,He, Xin-Hua. 2013

作者其他论文 更多>>

微信小程序