Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell-Entry Assay

文献类型： 外文期刊

第一作者： Ge, Jinying

作者： Ge, Jinying;Wen, Zhiyun;Wang, Xiyun;Hu, Sen;Liu, Yonggang;Kong, Xiangang;Chen, Hualan;Bu, Zhigao

作者机构：

关键词： SARS coronavirus;spike protein;VSV;pseudotype

期刊名称：IMPACT OF EMERGING ZOONOTIC DISEASES ON ANIMAL HEALTH

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收录情况： SCI

摘要： We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G~*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Δ G~*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSV Δ G~*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.

分类号： N

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