Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate

文献类型： 外文期刊

第一作者： Du, Meng

作者： Du, Meng;Chen, Jixiang;Zhang, Xiaohua;Li, Aijuan;Li, Yun;Wang, Yingeng

作者机构：

关键词： NON-CULTURABLE VIBRIO;SHIGELLA-DYSENTERIAE TYPE-1;STRESSED ESCHERICHIA-COLI;CAMPYLOBACTER-JEJUNI;IN-VIVO;STATE;RESUSCITATION;ENVIRONMENT;SURVIVAL;VULNIFICUS

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4 degrees C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (< 0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 10(9) CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity.

分类号： Q938

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