Gene expression in a starch synthase IIa mutant of barley: changes in the level of gene transcription and grain composition

文献类型: 外文期刊

第一作者: Clarke, B.

作者: Clarke, B.;Morell, M. K.;Jenkins, C. L. D.;Li, Z.;Liang, R.;Bird, A. R.

作者机构:

关键词: Barley;Endosperm;Microarray;SsIIa mutant

期刊名称:FUNCTIONAL & INTEGRATIVE GENOMICS ( 影响因子:3.41; 五年影响因子:3.616 )

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收录情况: SCI

摘要: The barley shrunken grain mutant M292 has a novel high-amylose starch phenotype caused by a mutation in the starch synthase IIa gene (SsIIa) located at the starch excess-6 (sex6) locus on chromosome 7H of barley. The loss of SSIIa enzyme activity leads to a decrease in amylopectin synthesis to less than 20% of the levels found in wild-type grains. Detailed composition analysis indicates that the contents of protein, non-starch polysaccharides, lipid, sucrose and hexoses, and fructo-oligosaccharides are increased in mature M292 grain compared to wild type. Using a microarray analysis, we characterize the differences between the transcription profiles of wild-type and mutant barley endosperms at mid-grain fill. The expression changes include genes involved in carbon storage, stress-related genes, and a number of transcripts with unassigned function. The changes in gene expression are discussed in terms of the altered grain composition of the mutant seed.

分类号: Q78

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