A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions

文献类型： 外文期刊

第一作者： Huang, Huoqing

作者： Huang, Huoqing;Luo, Huiying;Wang, Yaru;Fu, Dawei;Shao, Na;Wang, Guozeng;Yang, Peilong;Yao, Bin

作者机构：

关键词： gastric conditions;inorganic phosphorus;phytase;Yersinia rohdei;Y. pestis;SITE-DIRECTED MUTAGENESIS;SOYBEAN MEAL DIETS;MICROBIAL PHYTASE;SACCHAROMYCES-CEREVISIAE;PICHIA-PASTORIS;WEANLING PIGS;YOUNG-PIGS;PHOSPHORUS;THERMOSTABILITY;BIOAVAILABILITY

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Two novel phytase genes belonging to the histidine acid phosphatase family were cloned from Yersinia rohdei and Y. pestis and expressed in Pichia pastoris. Both the recombinant phytases had high activity at pH 1.5-6.0 (optimum pH 4.5) with an optimum temperature of 55 degrees C. Compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and a potential commercial phytase from Y. intermedia, the Y. rohdei phytase was more resistant to pepsin, retained more activity under gastric conditions, and released more inorganic phosphorus (two to ten times) from soybean meal under simulated gastric conditions. These superior properties suggest that the Y. rohdei phytase is an attractive additive to animal feed. Our study indicated that, in order to better hydrolyze the phytate and release more inorganic phosphorus in the gastric passage, phytase should have high activity and stability, simultaneously, at low pH and high protease concentration.

分类号： Q93

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