Molecular identification, polymorphism, and expression analysis of major histocompatibility complex class IIA and B genes of turbot (Scophthalmus maximus)

文献类型： 外文期刊

第一作者： Zhang, Yu-Xi

作者： Zhang, Yu-Xi;Chen, Song-Lin

作者机构：

关键词： Amino Acid Sequence;Animals;Base Sequence;Fish Diseases/genetics/microbiology;Fish Proteins/chemistry/genetics/metabolism;Flatfishes/*genetics;Gene Expression Profiling;Gene Expression Regulation;Genes;MHC Class II/*genetics;Molecular Sequence Data;Phylogeny;Polymorphism;Genetic;Vibrio Infections/metabolism/microbiology/veterinary

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) class II has a central role in the adaptive immune system by presenting foreign peptides to the T-cell receptor. The full lengths of MHC class II A and B cDNA were cloned from turbot by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE PCR), and genomic organization, molecular polymorphism, and expression of turbot class IIB gene were examined to study the function of class IIB gene in fish. The deduced amino acid sequence of turbot class II A (GenBank accession no.DQ001730) and turbot class IIB (GenBank accession no. DQ094170) had 69.8%, 67.6%, 65.5%, 59.2%, 54.5%, 52.8%, 46.2%, 46.6%, 28.3%, 28.5%, 22.2% identity and 71.5%, 70.7%, 67.1%, 68.4%, 46.7%, 53.5%, 46.7%, 50.0%, 25.2%, 29.2%, 27.6% identity with those of Japanese flounder, striped sea bass, red sea bream, cichlid, rainbow trout, Atlantic salmon, carp, zebrafish, nurse shark, mouse and human, respectively. Eleven class IIB alleles were identified from three turbot individuals. The amino acid sequence of turbot class IIB designated as Scma-DAB*0101 had 86.9%, 88.6%, 88.6%, 89.4%, 87.8%, 86.9%, 84.1%, 86.5%, 87.3%, 77.1%, and 86.9% identity with those of turbot class IIB 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 (Scma-DAB*0201- Scma-DAB*1201), respectively. Six different class IIB alleles observed in a single individual may infer the existence of three loci at least. Semiquantitative reverse transcriptase PCR (RT-PCR) demonstrated that turbot class IIA and B were ubiquitously expressed in normal tissues. Challenge of turbot with pathogenic bacteria, Vibrio anguillarum, resulted in a significant decrease in the expression of MHC class IIB mRNA from 24 h to 48 h after infection in liver and head kidney, and a significant decrease from 24 h to 72 h after infection in spleen, followed by an increase after 96 h, respectively.

分类号： Q81

  • 相关文献

[1]Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.). Chen, Ming,Xu, Zhaoshi,Xia, Lanqin,Li, Liancheng,Dong, Jianhui,Wang, Qiaoyan,Ma, Youzhi,Cheng, Xianguo.

[2]Disruption effect of Microplitis bicoloratus polydnavirus EGF-like protein, MbCRP, on actin cytoskeleton in lepidopteran insect hemocytes. Luo, Kai-Jun,Pang, Yi.

[3]Apoptosis Induced by Duck Reovirus p10.8 Protein in Primary Duck Embryonated Fibroblast and Vero E6 Cells. Geng, Hongwei,Zhang, Yun,Guo, Dongchun,Wang, Yu,Liu, Ming,Zhang, Yun,Liu-Partanen, Yin,Tong, Guangzhi.

[4]Structure and Regulation of the gab Gene Cluster, Involved in the gamma-Aminobutyric Acid Shunt, Are Controlled by a sigma(54) Factor in Bacillus thuringiensis. Song, Fuping,Jiang, Yanan,Sun, Changpo,Zhang, Jie,Zhu, Li,Sun, Changpo,Huang, Dafang,Peng, Qi.

[5]A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris. Luo, Huiying,Huang, Huoqing,Yang, Peilong,Wang, Yaru,Yuan, Tiezheng,Wu, Ningfeng,Yao, Bin,Fan, Yunliu.

[6]A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhang, Qi,Lei, Cai-Lin,Zhang, Shi-Hong.

[7]Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Ueda, Mami,Fujisaki, Kozo,Zhou, Jinlin,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo.

[8]A new method for SNP discovery. Xu, Jian-Yong,Xu, Gen-Bo,Chen, Song-Lin,Xu, Jian-Yong,Xu, Gen-Bo.

[9]Prolactin receptor as a candidate gene for prolificacy of small tail han sheep. Chu, M. X.,Mu, Y. L.,Fang, L.,Ye, S. C.,Sun, S. H..

[10]Development of silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) genetic maps using microsatellite and AFLP markers and a pseudo-testcross strategy. Liao, M.,Zhang, L.,Yang, G.,Zhu, M.,Wang, D.,Wei, Q.,Zou, G.,Chen, D..

[11]Unique sequence characteristics of genes in the leftmost region of unique long region in duck enteritis virus. Liu, Xiaoli,Liu, Shengwang,Li, Huixin,Han, Zongxi,Shao, Yuhao,Kong, Xiangang.

[12]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[13]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[14]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[15]Characterization of seed proteome in Brachypodium distachyon. Wang, Ke,Han, Xiaofeng,Dong, Kun,Gao, Liyan,Yan, Yueming,Wang, Ke,Han, Xiaofeng,Li, Hongyu,Ye, Xingguo,Ma, Wujun.

[16]Proteomic analysis of PEG-simulated drought stress-responsive proteins of rice leaves using a pyramiding rice line at the seedling stage. Xiong, Jian-Hua,Li, Yang-Sheng,Xiong, Jian-Hua,Fu, Bin-Ying,Xu, Hua-Xue.

[17]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[18]Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia. Matsumoto, Yukiko,Sato, Yuki,Noda, Hiroaki,Fu, Qiang,Matsumura, Masaya.

[19]A 90-day safety study of genetically modified rice expressing rhIGF-1 protein in C57BL/6J rats. Tang, Maoxue,Cheng, Wenke,Qian, Lili,Yang, Shulin,Cui, Wentao,Li, Kui,Tang, Maoxue,Cheng, Wenke,Qian, Lili,Yang, Shulin,Cui, Wentao,Li, Kui,Xie, Tingting,Yang, Daichang.

[20]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

作者其他论文 更多>>

微信小程序