Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties

文献类型: 外文期刊

第一作者: Zhao, Li-Ping

作者: Zhao, Li-Ping;Liu, Zhen;Chen, Liang;Yao, Ming-Zhe;Wang, Xin-Chao

作者机构:

关键词: expressed sequence tag;germplasm;microsatellite;SSRs;tea plant;EXPRESSED SEQUENCE TAGS;GERMPLASM;MARKERS;GENOME;GENES;L.

期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A total of 31 expressed sequence tag (EST)derived polymorphic microsatellites from tea plant, Camellia sinensis (L.) O. Kuntze, were generated and characterized using the ESTs of the author's EST sequencing project and other sources. A set of 40 accessions tea germplasms had been used to examine the diversity. Among the 31 microsatellite loci, 24 had two to eight polymorphic alleles. Observed heterozygosity (H,,) were relatively higher (on an average of 0.533), varying from 0.175 (primer 21) to 0.950 (primer 228). Cross-species polymorphic amplification in other four species and two varieties of section Thea (L.) Dyer genus Camellia L. was successful for the 24 loci. Contribution of the 24 novel EST-SSR primers presented here will provide necessary and powerful molecular tools for management and conservation studies on the tea germplasms in the future.

分类号: Q1

  • 相关文献

[1]Isolation and characterization of microsatellite loci from the mulberry, Morus L.. Zhao, WG,Mia, XX,Jia, SH,Pan, YL,Huang, Y.

[2]Genotypic variation of beta-carotene and lutein contents in tea germplasms, Camellia sinensis (L.) O. Kuntze. Wang, Xin-Chao,Chen, Liang,Ma, Chun-Lei,Yao, Ming-Zhe,Yang, Ya-Jun. 2010

[3]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[4]Isolation and biological characterization of tendon-derived stem cells from fetal bovine. Yang, Jinjuan,Huang, Hongmei,Liu, Yingjie,Yang, Jinjuan,Zhao, Qianjun,Wang, Kunfu,Liu, Hao,Ma, Caiyun.

[5]Two novel loci for pollen sterility in hybrids between the weedy strain Ludao and the Japonica variety Akihikari of rice (Oryza sativa L.). Jing, Wen,Zhang, Wenwei,Jiang, Ling,Chen, Liangming,Zhai, Huqu,Wan, Jianmin.

[6]Isolation and characterization of 16 polymorphic microsatellite markers for the mudskipper (Boleophthalmus pectinirostris). Wang, Cong-Tao,Liu, Zhi-Zhi,Zhang, Jia-Mei,Zeng, Zhen,Tang, Wen-Qiao,Ma, Ling-Bo,He, An-Yuan,Tang, Shou-Jie.

[7]A preliminary study on genetic analysis and purity assessment of the garlic germplasm and seed bulbs by the "finger printing" technique. Xu, PW,Yang, CL,Qu, SS,Yang, CY. 2005

[8]Differential growth response and carbohydrate metabolism of global collection of perennial ryegrass accessions to submergence and recovery following de-submergence. Yu, Xiaoqing,Jiang, Yiwei,Luo, Na,Yan, Jiapei,Tang, Jinchi,Liu, Shuwei. 2012

[9]The effect of low water content on seed longevity. Hu, CL,Zhang, YL,Tao, M,Hu, XR,Jiang, CY. 1998

[10]Genetic Diversity Revealed by Fluorescent-AFLP Markers for Apricot Germplasms in Shandong, China. Yuan, Z. H.,Yin, Y. L.,Feng, L. J.,Zhao, X. Q.,Wang, J. Z.,An, G. N.,Zhang, C. Y.. 2011

[11]Peach germplasm and breeding programs at Zhengzhou in China. Wang, LR,Zhu, GR,Fang, WC. 2002

[12]Diversity evaluation of wheat germplasm resources in China. Li, LH,Zheng, DS,Yang, XM,Li, XQ,Liu, SC,Song, CH. 1998

[13]The advancement of research on litchi and longan germplasm resources in China. Wu, Yuanli,Yi, Ganjun,Zhou, Birong,Zeng, Jiwu,Huang, Yonghong. 2007

[14]Diversity of Sicilian broccoli (Brassica oleracea var. italica) and cauliflower (Brassica oleracea var. botrytis) landraces and their distinctive bio-morphological, antioxidant, and genetic traits. Branca, Ferdinando,Chiarenza, Giuseppina Laura,Cavallaro, Chiara,Tribulato, Alessandro,Gu, Honghui,Zhao, Zhenqing. 2018

[15]Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers. Chang Yumei,Ding Lei,Liang Liqun,Chang Yumei,He Jianguo,Ding Lei,Li Mingyun,Xue Liangyi,Liang Liqun,Lei Qingquan. 2008

[16]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[17]Short- and long-term effects of ultra-drying on germination and growth of vegetable seeds. Shen, D,Qi, XQ. 1998

[18]The genetic diversity of annual wild soybeans grown in China. Dong, YS,Zhuang, BC,Zhao, LM,Sun, H,He, MY. 2001

[19]Observing and evaluating of the introduced onion varieties and lines from abroad. Qu, SS,Xu, PW,Sun, JB. 2005

[20]Cytogenetics and germplasm enrichment in Brassica allopolyploids in China. Li Zai-yun,Wang You-ping,Wang You-ping. 2017

作者其他论文 更多>>