Small-scale Expressed Sequence Tag Analysis Of Theileria Uilenbergi: Identification Of A Gene Family Encoding Potential Antigenic Proteins

文献类型： 外文期刊

第一作者： Liu, Zhijie

作者： Liu, Zhijie;Ahmed, Jabbar S.;Seitzer, Ulrike;Liu, Zhijie;Dang, Zhisheng;Luo, Jianxian;Yin, Hong

作者机构：

关键词： theileria uilenbergi;merozoite;cdna library;sequencing

期刊名称：Animal Biodiversity and Emerging Diseases: Prediction and Prevention

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收录情况： SCI

摘要： Recently, Theileria sp. (China) has been designated as T. luwenshuni [formerly Theileria sp. (China 1)] and T uilenbergi [formerly Theileria sp. (China 2)]. A cDNA library of T. uilenbergi merozoites was constructed and subjected to random sequencing. Among the obtained sequences were three highly identical cDNA clones, indicating a gene family. Bioinformatic analyses indicated these genes contain signal peptides and encode potential immunogenic proteins. The presence of tandemly arranged and additional variants of these genes was shown. Analysis of one recombinantly expressed clone revealed immunoreactivity for serum from Theileria-infected animals. No cross-reaction with serum of T. lestoquardi-, Babesia motasi-, or Anaplasma ovis-infected animals was observed, indicating a potential antigen for development of serological diagnostic tools.

分类号： N

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