Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius

文献类型： 外文期刊

第一作者： Yuan, Tiezheng

作者： Yuan, Tiezheng;Yang, Peilong;Wang, Yaru;Meng, Kun;Luo, Huiying;Yao, Bin;Zhang, Wei;Wu, Ningfeng;Fan, Yunliu

作者机构：

关键词： expression;beta-Galactosidase;lactose;Pichia pastoris;thermostability;LACTOSE;PURIFICATION

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A genomic DNA library screen yielded the nucleotide sequence of a 12 kb fragment containing a gene (2067 bp) coding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius ATCC 27009. The beta-galactosidase gene was expressed in Pichia pastoris, and up to 90 mg recombinant beta-galactosidase/l accumulated in shake flask cultures. Using o-nitrophenyl-beta-D-galactopyranoside as a substrate, the optimum pH and temperature of the purified recombinant beta-galactosidase were 5.8-6.0 and 70 degrees C, respectively. The enzyme retained 90% of its activity when heated at 70 degrees C for 30 min. Approximately 48% of lactose in milk was hydrolyzed following treatment with the recombinant enzyme over 60 min at 65 degrees C.

分类号： Q81

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