Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper

文献类型: 外文期刊

第一作者: Tang, KX

作者: Tang, KX;Hu, QN;Sun, XF;Wan, BL;Qi, HX;Lu, XG

作者机构:

关键词: cotransformation;Oryza sativa L;particle bombardment;segregation analysis;snowdrop lectin gene (gna);transgenic rice

期刊名称:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT ( 影响因子:2.252; 五年影响因子:2.139 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectahle marker hygromycin phosphotransferase gene (hpt), the reporter #bate#-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. Thirty-five independent transgenic rice plants were regenerated from 177 bombarded calluses. Eighty-three percent of the transgenic plants contained all three genes, as revealed by Southern blot analysis. Western blot analysis revealed that 23 out of 29 gna-containing transgenic plants expressed Galanthus nivalis agglutinin (GNA) (79 percent) at various levels with the highest expression being approximately 0.5 percent of total soluble protein. Genetic analysis confirmed Mendelian segregation of all three transgenes (gna, hpt and gusA) in the R2 progeny. Amongst the R2 generation, two independent homozygous lines were identified that expressed all three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing the survival, overall fecundity of BPH, retarding development, and decreasing the feeding ofBPH. These BPH-resistant lines have been incorporated into a rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH.

分类号: Q94

  • 相关文献

[1]Transgenic fertile japonica rice plants expressing a modified crylA(b) gene resistant to yellow stem borer. Wu, C,Fan, Y,Zhang, C,Oliva, N,Datta, SK. 1997

[2]Mixed inheritance model for resistance to agromyzid beanfly (Melanagromyza sojae Zehntner) in soybean. Wang, JK,Gai, JY. 2001

[3]Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Zhi-Hong Liu,Hong-Mei Wang,Chang-Bing Yao.

[4]Creation of transgenic bananas expressing human lysozyme gene for Panama wilt resistance. Pei, XW,Chen, SK,Wen, RM,Ye, S,Huang, JQ,Zhang, YQ,Wang, BS,Wang, ZX,Jia, SR. 2005

[5]Efficient regeneration and genetic transformation platform applicable to five Musa varieties. Liu, Juhua,Gao, Pengzhao,Zhang, Jing,Jia, Caihong,Zhang, Jianbin,Hu, Wei,Xu, Biyu,Jin, Zhiqiang,Sun, Peiguang,Wang, Jiashui,Jin, Zhiqiang,Sun, Xiuxiu. 2017

[6]Introduction of cecropin B gene into rice (Oryza sativa L) by particle bombardment and analysis of transgenic plants. Huang, DN,Zhu, B,Yang, W,Xue, R,Xiao, H,Tian, WZ,Li, LC,Dai, SH. 1996

[7]IDENTIFICATION OF A SET OF RFLP PROBES FOR SUBSPECIES DIFFERENTIATION IN ORYZA-SATIVA L. QIAN, HR,ZHUANG, JY,LIN, HX,LU, J,ZHENG, KL. 1995

[8]Genome-Wide Disruption of Gene Expression in Allopolyploids but Not Hybrids of Rice Subspecies. Xu, Chunming,Bai, Yan,Zhao, Na,Hu, Lanjuan,Liu, Bao,Xu, Chunming,Wendel, Jonathan F.,Lin, Xiuyun,Zhao, Na,Gong, Zhiyun. 2014

[9]QTLs influencing panicle size detected in two reciprocal introgressive line (IL) populations in rice (Oryza sativa L.). Mei, HW,Xu, JL,Li, ZK,Yu, XQ,Guo, LB,Wang, YP,Ying, CS,Luo, LJ. 2006

[10]YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice. Zhang, Fantao,Luo, Xiangdong,Xie, Jiankun,Hu, Biaolin,Wan, Yong. 2013

[11]Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice. Fan, Xiaolei,Wu, Jiemin,Chen, Taiyu,Chen, Hao,Zhou, Fei,Lin, Yongjun,Fan, Xiaolei,Wu, Jiemin,Chen, Taiyu,Chen, Hao,Zhou, Fei,Lin, Yongjun,Tie, Weiwei. 2015

[12]Yield performances of japonica introgression lines selected for drought tolerance in a BC breeding programme. He, Y. X.,Zheng, T. Q.,Wang, L. F.,Gao, Y. M.,Zhai, H. Q.,Xu, J. L.,Zhu, L. H.,Li, Z. K.,He, Y. X.,Xu, Z. J.,He, Y. X.,Hao, X. B.,Hua, Z. T.,Li, Z. K.. 2010

[13]LEAFY HEAD2, which encodes a putative RNA-binding protein, regulates shoot development of rice. Xiong, Guo Sheng,Hu, Xing Ming,Jiao, Yong Qing,Yu, Yan Chun,Chu, Cheng Cai,Li, Jia Yang,Qian, Qian,Wang, Yong Hong. 2006

[14]Identifying different types of dedifferentiated microspores from indica-japonica F-1 hybrids with subspecies-differentiating RFLP probes in rice. Xie, JH,Lu, J,Zhuang, JY,Lin, HX,Qian, HR,Gao, MW,Zheng, KL. 1997

[15]Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. Wang, Ningning,Zhang, Di,Wang, Zhenhui,Xun, Hongwei,Wang, Hui,Huang, Wei,Liu, Ying,Li, Ning,Ou, Xiufang,Zhang, Chunyu,Liu, Bao,Wang, Ningning,Ma, Jian,Lin, Xiuyun,Zhang, Chunyu,Wang, Ningning,Wang, Ming-Bo. 2014

[16]Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling. Wang, Wen-Sheng,Zhao, Xiu-Qin,Li, Min,Huang, Li-Yu,Xu, Jian-Long,Zhang, Fan,Cui, Yan-Ru,Fu, Bin-Ying,Li, Zhi-Kang,Li, Min,Xu, Jian-Long,Fu, Bin-Ying,Li, Zhi-Kang.

[17]Large-scale production of enhancer trapping lines for rice functional genomics. Yang, YZ,Peng, H,Huang, HM,Wu, JX,Ha, SR,Huang, DF,Lu, TG.

[18]Genetic mapping of a QTL controlling source-sink size and heading date in rice. Cheng, Shihua.

[19]Construction of chromosomal segment substitution lines and genetic dissection of introgressed segments associated with yield determination in the parents of a super-hybrid rice. Liu, Xi,Zhao, Zhigang,Liu, Linglong,Xiao, Yinghui,Tian, Yunlu,Liu, Shi-Jia,Chen, Liangming,Wang, Yihua,Liu, Yuqiang,Chen, Saihua,Zhang, Wenwei,Wang, Chunming,Jiang, Ling,Wan, Jianmin,Wan, Jianmin.

[20]Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight. Wang, Xu-Ming,Zhou, Jie,Yang, Yong,Yu, Fei-Bo,Chen, Juan,Yu, Chu-Lang,Wang, Fang,Cheng, Ye,Yan, Cheng-Qi,Chen, Jian-Ping,Yu, Fei-Bo,Chen, Juan. 2013

作者其他论文 更多>>