Molecular cloning and characterization of the Myf5 gene in sea perch (Lateolabrax japonicus)

文献类型： 外文期刊

第一作者： Ye, Han-Qing

作者： Ye, Han-Qing;Chen, Song-Lin;Xu, Jian-Yong

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关键词： sea perch;Lateolabrax japonicas;Myf5;muscle;gene expression;MUSCLE-SPECIFIC EXPRESSION;SKELETAL-MUSCLE;PROMOTER ANALYSIS;ZEBRAFISH;MYOD;MYOGENESIS;TRANSCRIPTION;PATTERNS;SEQUENCE;CELLS

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

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收录情况： SCI

摘要： The cDNA of myogenic factor (Myf5) was isolated from sea perch (Lateolabrax japonicas) using Reverse-transcription Polymerase Chain Reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The 5' flanking sequence of the cDNA contains a TATA box, GC box, CAAT box, several E box sites and muscle-specific regulatory elements determined by genome walking. The Myf5 gene consists of 3 exons and 2 introns. The open reading frame was found to code a protein with 238 amino-acid residues, containing the conserved basic helix-loop-helix domain (bHLH). RT-PCR indicated the Myf5 was highly expressed in muscle, and weakly expressed in brain, eyes, spleen, gill, liver, kidney, intestine and heart. In early embryonic stages, Myf5 mRNA transcripts are highly detectable in the early gastrula stage while decreasing up to a low level at the late gastrula stage, subsequently greatly increased up to the highest level in the somites stage, then gradually decreases from the tail-bud stage to 15 d larvae after hatching, but they are still detectable. Further, Myf5 mRNA was expressed in several sea perch cell lines such as LJES1, LJHK, LJH-1, LJH-2, US, LJL, although its expression level varied greatly among different cell lines. (c) 2007 Elsevier Inc. All rights reserved.

分类号： Q5

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