Isolation and characterization of microsatellite loci from the mulberry, Morus L.

文献类型： 外文期刊

第一作者： Zhao, WG

作者： Zhao, WG;Mia, XX;Jia, SH;Pan, YL;Huang, Y

作者机构：

关键词： mulberry;molecular markers;microsatellite;simple-sequence repeat;SSRs;GENETIC DIVERSITY;MARKERS;GERMPLASM

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Development of inicrosatellite markers for mulberry, Morus L. was performed according to the strong affinity between biotin and streptavidin. The method included ligation of 400-1000 bp enzyme-digested fragments and adaptor, affinity capture of microsatellite repeat using biotinylated oligoprobe attached to Dynabeads M-280 streptavidin beads. A (CA)(15) enriched microsatellite library was constructed for mulberry. After sequence analysis on 96 randomly picked positive colonies, 24 (25%) of the colonies were found to contain microsatellite sequences, and 20 primer pairs were designed. For polymorphism assessment, 27 mulberry genotypes were checked and 10 of the primers tested were shown to be polymorphic. The novel polymorphic microsatellites produced an average of 4.9 alleles/locus, an average observed heterozygosity of 0.4296 and an average PIC (polymorphism information content) value of 0.4483. Cluster analysis using microsatellite markers was further carried out on the matrix of similarity coefficient with UPGMA method and the phenogram was constructed. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

分类号： Q94

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