Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects

文献类型： 外文期刊

第一作者： Liang, Gemei

作者： Liang, Gemei;Li, Haitao;He, Kanglai;Song, Fuping;Feng, Xue;Zhang, Jie;Crickmore, Neil;Huang, Dafang

作者机构：

关键词： Bacillus thuringiensis;novel cry1Ah gene;insecticidal activity;Ostrinia furnacalis;Helicoverpa armigera;Chilo suppressalis;HELICOVERPA-ARMIGERA LEPIDOPTERA;CRYSTAL PROTEIN GENE;DELTA-ENDOTOXIN;INSECTICIDAL ACTIVITY;RESISTANCE;IDENTIFICATION;EXPRESSION;NOCTUIDAE;BOLLWORM;STRAINS

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain BT8. The toxin expressed in HD73(-) exhibited high toxicity against lepidopteran larvae of Ostrinia furnacalis, Helicoverpa armigera, Chilo suppressalis, and Plutella xylostella. The 50% lethal concentrations (LC(50)s) were 0.05, 1.48, 0.98 mu g g(-1) and 1.52 mu g mL(-1), respectively. The LC(50)s of Cry1Ah were significantly lower than that of Cry1Ac for H. armigera, C. suppressalis, and O. furnacalis, and lower than that of Cry1Ab and Cry1Ie for Ostrinia furnacalis. The high toxicity against a range of pest species makes this novel toxin a potential candidate for insect biocontrol.

分类号： Q93

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