Medium Optimization for Enzymatic Production of L-Cysteine by Pseudomonas sp Zjwp-14 Using Response Surface Methodology

文献类型： 外文期刊

第一作者： Lv, Guo-Ying

作者： Lv, Guo-Ying;Wang, Pu;He, Jun-Yao;Lv, Guo-Ying;Li, Xiao-Nian

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关键词： medium optimization

期刊名称：FOOD TECHNOLOGY AND BIOTECHNOLOGY （ 影响因子：3.918； 五年影响因子：3.71 ）

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收录情况： SCI

摘要： Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-Delta(2)-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzyme activity for biotransformation of DL-ATC to L-cysteine. In order to investigate the quantitative effects for four variables selected from Plackett-Burman design on enzyme activity,) a central composite design was subsequently employed for further optimization. The determination coefficient (R-2) was 0.9817, and the results show that the regression models adequately explain the data variation and represent the actual relationships between the parameters and responses. The optimal medium for Pseudomonas sp. Zjwp-14 was composed of (in g/L): glycerol 16.94, DL-ATC 4.59, yeast extract 6.99, NaCl 5.0, peptone 5.0, beef extract 5.0, MgSO4 center dot 7H(2)O 0.4, and pH=7.94. Under the optimal conditions, the maximum intracellular enzyme activity of 918.7 U/mL in theory and 903.6 U/mL in the experiment were obtained, with an increase of 15.6 % compared to the original medium components. In a 5-litre fermentor, cultivation time for Pseudomonas sp. Zjwp-14 was cut down for 6 h and the maximum enzyme activity reached 929.6 U/mL.

分类号： TS2

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