Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana
文献类型: 外文期刊
第一作者: Sheng, Xiaoguang
作者: Sheng, Xiaoguang;Liu, Fan;Zhu, Yuelin;Zhao, Hong;Zhang, Li;Chen, Bin
作者机构:
关键词: brassica oleracea;matthiola incana;intergeneric hybridization;hybridity verification;SINAPIS-ALBA L;PROTOPLAST FUSION;ORYCHOPHRAGMUS-VIOLACEUS;NAPUS L;B-CAMPESTRIS;HYBRIDIZATION;CYTOGENETICS;SATIVA;ACID
期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The gene pool of Brassica oleracea was enriched via intergeneric somatic hybridization between B. oleracea (2n = 18) and Matthiola incana (2n = 14). One hundred and eighteen plants were obtained from 96 calli. Only four plants (H1, H2, H3 and H4) showed an intermediate phenotype from the parents; among these, H1 and H3 arose from the same callus. Random amplified polymorphic DNA (RAPD), sequence-related amplified polymorphism (SRAP), and cytological analyses confirmed that H1 and H3 were hybrids. The nuclear DNA content of the regenerated plants was determined by flow cytometry. More than half of the plants contained a nuclear DNA content of 1.3 to 3.9 pg/cell, which was higher than the content of B. oleracea but lower than that of M. incana. H1 contained 4.89 +/- 0.02 pg of DNA per cell, while H3 nuclear DNA content was estimated at 4.87 +/- 0.06 pg/cell. Cytological study of the root-tip cells revealed that the majority of the H1 and H3 hybrid cells contained 28 chromosomes.
分类号: Q942
- 相关文献
作者其他论文 更多>>
-
Pathogenicity of Bipolaris oryzae isolated from banana to different plant species
作者:Zhang, Zhaojing;Zhao, Hong;Zhang, Zhaojing;Zhao, Hong;Ouyang, Yanfei;Zhang, Xin;Qi, Yanxiang;Ouyang, Yanfei
关键词:
Bipolaris oryzae ; Host range; Identification; In vitro inoculation -
Fusarium spp. Associated With Leaf Blight of Hosta (Hosta plantaginea L.) in China
作者:Jiao, Chenyang;Zhang, Li;Xu, Xi;Yang, Xilang;Guo, Lifeng;Wang, Xiangjing;Xiang, Wensheng;Zhao, Junwei;Guo, Lifeng;Xiang, Wensheng
关键词:
Fusarium spp.; Hosta; pathogenicity test; phylogenetic analysis -
Konjac glucomannan: A key ingredient for controlling fibrous structure formation in walnut protein and wheat gluten meat analogues during the high-moisture extrusion process
作者:Lei, Shuwen;Zhao, Chunyan;Zhao, Hong;Fu, Xiaoping;Gong, Jiashun;Gong, Jiashun;Peng, Chunxiu;Miao, Yue
关键词:Walnut protein; Wheat gluten; Konjac glucomannan; Meat analogues; Fibrous structure formation; High-moisture extrusion process
-
Alteration in Tracheal Morphology and Transcriptomic Features in Calves After Infection with Mycoplasma bovis
作者:Liu, Fan;Yang, Fei;Guo, Lei;Yang, Mengmeng;Li, Jidong;He, Shenghu;Liu, Fan;Guo, Yanan;Li, Yong
关键词:Mycoplasma bovis; trachea; transcriptome; signaling pathway; IL-6
-
The nitrogen supply capacity and application methods of straw-chemical mixed fertilizer in the sweet corn variety 'Zhetian 19'
作者:Hou, Junfeng;Chen, Bin;Zhang, Ping;Tan, Heping;Han, Hailiang;Bao, Fei;Zhao, Fucheng;Wang, Yanli
关键词:SCMF; Sweet corn; Yield; Chlorophyll content; Dry matter; Soil nutrient
-
Theabrownin remodels the circadian rhythm disorder of intestinal microbiota induced by a high-fat diet to alleviate obesity in mice
作者:Zhao, Chunyan;Lei, Shuwen;Zhao, Hong;Li, Zelin;Miao, Yue;Gong, Jiashun;Li, Zelin;Gong, Jiashun;Peng, Chunxiu;Miao, Yue
关键词:
-
TaFAR9 and TaFAR10 synergistically regulate fertility conversion of photo-thermo-sensitive genic male sterility lines in wheat by modulating ROS homeostasis
作者:Niu, Fuqiang;Geng, Qiang;Hu, Lingling;Zhang, Li;Gao, Xiaoran;Liu, Jinke;Song, Xiyue;Liu, Zihan;Bai, Jianfang;Liu, Yongjie;Yuan, Shaohua;Zhai, Nuo;Zhao, Changping;Zhang, Liping
关键词:Photo-thermo-sensitive genic male sterility; Fatty acyl CoA reductase; Fertility conversion; Reactive oxygen species; Wheat