Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana

文献类型: 外文期刊

第一作者: Sheng, Xiaoguang

作者: Sheng, Xiaoguang;Liu, Fan;Zhu, Yuelin;Zhao, Hong;Zhang, Li;Chen, Bin

作者机构:

关键词: brassica oleracea;matthiola incana;intergeneric hybridization;hybridity verification;SINAPIS-ALBA L;PROTOPLAST FUSION;ORYCHOPHRAGMUS-VIOLACEUS;NAPUS L;B-CAMPESTRIS;HYBRIDIZATION;CYTOGENETICS;SATIVA;ACID

期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )

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收录情况: SCI

摘要: The gene pool of Brassica oleracea was enriched via intergeneric somatic hybridization between B. oleracea (2n = 18) and Matthiola incana (2n = 14). One hundred and eighteen plants were obtained from 96 calli. Only four plants (H1, H2, H3 and H4) showed an intermediate phenotype from the parents; among these, H1 and H3 arose from the same callus. Random amplified polymorphic DNA (RAPD), sequence-related amplified polymorphism (SRAP), and cytological analyses confirmed that H1 and H3 were hybrids. The nuclear DNA content of the regenerated plants was determined by flow cytometry. More than half of the plants contained a nuclear DNA content of 1.3 to 3.9 pg/cell, which was higher than the content of B. oleracea but lower than that of M. incana. H1 contained 4.89 +/- 0.02 pg of DNA per cell, while H3 nuclear DNA content was estimated at 4.87 +/- 0.06 pg/cell. Cytological study of the root-tip cells revealed that the majority of the H1 and H3 hybrid cells contained 28 chromosomes.

分类号: Q942

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