Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues

文献类型： 外文期刊

第一作者： Zhao, Yinli

作者： Zhao, Yinli;Zhang, Gaiping;Liu, Qingtang;Teng, Man;Yang, Jifei;Zhao, Yinli;Wang, Jianhua

作者机构：

关键词： Enrofloxacin;test strip;colloidal gold;immunoassay;rapid test

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination of enrofloxacin (ENR) residues in chicken muscles. For this purpose, a specific monoclonal antibody (mAb) for ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using ENR standards prepared in chicken muscle extracts from 0 to 24.3 ng/mL (μg/ kg), the method indicated that the detection limit of the test strip, as measured in a strip scanner, was as low as 0.138 μg/kg of ENR and the half-maximal inhibition concentration (IC_(50)) was 0.935 μg/kg. For samples spiked at 10, 20, and 30 μg/kg, the recovery was between 85.3 and 96.1% and the coefficient of variation [CV (%)] was between 4.5 and 7.91%. Parallel analysis of muscle samples from chickens fed ENR showed good comparable results obtained from the test strip and LC-MS. Each test requires 5-10 min. The data indicate that the method has high sensitivity, specificity, and the advantages of simplicity and speed of performance. Therefore, the test strip provides a useful screening method for quantitative, semiquantitative, or qualitative detection of ENR residues in chicken muscles.

分类号： S13

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