Construction and Characterization of a Bacterial Artificial Chromosome Library for Triticum boeoticum

文献类型： 外文期刊

第一作者： Chen, FG

作者： Chen, FG;Zhang, XY;Xia, GM;Jia, JZ

作者机构：

关键词： triticum boeoticum;BAC library;genome

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss (A~bA~b) using the bacterial artificial chromosome (BAC) vector pECBAC1. The library consists of about 170 000 clones. A random sampling analysis of 200 BAC clones indicates that the average insert size is 104 kb. Based on the genome size of T. boeoticum, the library is about three times as large as T. boeoticum hap-loid genome (5 600 Mb) . Screening the BAC library with cpDNA sequence psbA gene and mtDNA sequence atp6 gene as probe shows that contamination of the library with chloroplast and mitochondrial clones is less than 1%. The library will be a useful platform in gene clone and genomic research of wheat.

分类号： Q94

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