Proteomic Analysis of Royal Jelly from Three Strains of Western Honeybees (Apis mellifera)

文献类型： 外文期刊

第一作者： Li, Jianke

作者： Li, Jianke;Wang, Ting;Zhang, Zhaohui;Pan, Yinghong

作者机构：

关键词： Royal jelly (RJ);major royal jelly protein (MRJP);honeybee (Apis mellifera);proteome

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To compare the protein complement of royal jelly (RJ) from high RJ producing honeybees (Apis mellifera L.), a strain of A. mellifera artificially selected for increased RJ production from Italian honeybees in China for more than two decades was compared to those of native Italian honeybees (A. mellifera L.) and Carnica honeybees (A: mellifera C); the protein in RJ from these three strains of honeybees was partially identified by using a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS), and a protein engine identification tool applied to the honeybee genome. The results showed that 152, 157, and 137 proteins were detected in the three species of RJ; among which 57, 57, 51 high abundant proteins ere identified, respectively. Most identifited spots, 45, 45, 41, were assigned to major royal jelly proteins (MRJPs). Remarkable differences were found in the heterogeneity of the MRJPs, in particular, MRJP3. Also, 3-glucose oxidase, 1-peroxiredoxin (PRDX), and 1-glutathione S-transferase (GST) S1 were identified in three RJ samples. Furthermore, during the determination of the peptides mass fingerprinting (PMF) of each spot, for the first time, PRDX and GST S1 proteins have been identified in RJ. Thus, the results suggest that the protein complement of high RJ producing honeybees is not different compared to native Italian honeybees, while a difference remains between Carnica honeybees.

分类号： S13

  • 相关文献

[1]Comparative Analyses of Proteome Complement Between Worker Bee Larvae of High Royal Jelly Producing Bees (A. m. ligustica) and Carniolian Bees (A. m. carnica). Chen Jian,Li Jian-ke. 2009

[2]Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation. Kang Liu,Meiling Han,Chaojun Zhang,Liangyu Yao,Jing Sun,Tianzhen Zhang.

[3]iTRAQ-Based Comparative Proteomic Analysis of Seedling Leaves of Two Upland Cotton Genotypes Differing in Salt Tolerance. Wenfang Gong,Feifei Xu,Du, Xiongming,Junling Sun,Zhen Peng,Shoupu He,Zhaoe Pan,Xiongming Du. 2017

[4]iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.). Ji Liu,Chaoyou Pang,Hengling Wei,Meizhen Song,Yanyan Meng,Jianhui Ma,Shuli Fan,Shuxun Yu.

[5]Proteomic analysis of mycelial proteins from Magnaporthe oryzae under nitrogen starvation. Zhou, X. -G.,Zhao, Z. -W.,Zhou, X. -G.,Yu, P.,Dong, C.,Yao, C. -X.,Ding, Y. -M.,Tao, N.,Zhou, X. -G.,Yu, P.,Dong, C.,Yao, C. -X.,Ding, Y. -M.,Tao, N.. 2016

[6]Comparative transcriptome and proteome profiling of two Citrus sinensis cultivars during fruit development and ripening. Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li,Wang, Jian-hui,Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li,Wang, Jian-hui,Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li. 2017

[7]Transcriptomic and proteomic analysis of Locusta migratoria eggs at different embryonic stages: Comparison for diapause and non-diapause regimes. Hao Kun,Wang Jie,Tu Xiong-bing,Zhang Ze-hua,Whitman, Douglas W.. 2017

[8]Proteomics Dissection of Cold Responsive Proteins Based on PEG Fractionation in Arabidopsis. Wang Shang,Xi Jinghui,Li Shanyu,Liu Xiangguo,Hao Dongyun. 2014

[9]Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid. Zhong, Weijing,Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Zhong, Weijing,Yang, Lin,Zou, Shutong. 2011

[10]Proteomic analysis of responsive root proteins of Fusarium oxysporum-infected watermelon seedlings. Zhang, Man,Xu, Jinhua,Liu, Guang,Yao, Xiefeng,Ren, Runsheng,Yang, Xingping. 2018

[11]Modulation of protein expression in alfalfa (&ITMedicago sativa&IT L.) root and leaf tissues by &ITFusarium proliferatum&IT. Cong Li-li,Long Rui-cai,Kang Jun-mei,Zhang Tie-jun,Wang Zhen,Yang Qing-chuan,Sun Yan,Li Ming-na,Cong Li-li. 2017

[12]Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing. Cheng, GF,Lin, JJ,Feng, XG,Fu, ZQ,Jin, YM,Yuan, CX,Zhou, YC,Cai, YM. 2005

[13]Changes in the proteomic profiles of mouse brain after infection with cyst-forming Toxoplasma gondii. Zhou, Dong-Hui,Zhao, Fu-Rong,Huang, Si-Yang,Xu, Min-Jun,Song, Hui-Qun,Zhu, Xing-Quan,Su, Chunlei,Zhu, Xing-Quan. 2013

[14]Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds. Xu, Heng-Heng,Liu, Shu-Jun,Wang, Wei-Qing,Song, Song-Quan,Li, Ni,Moller, Ian M.. 2016

[15]Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua. 2017

[16]A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.). Hou, Yuxuan,Qiu, Jiehua,Wang, Yifeng,Li, Zhiyong,Zhao, Juan,Tong, Xiaohong,Lin, Haiyan,Zhang, Jian,Lin, Haiyan. 2017

[17]Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae. Zhao, Fu'an,Fang, Weiping,Xie, Deyi,Zhao, Yuanming,Tang, Zhongjie,Li, Wu,Nie, Lihong,Lv, Shuping,Zhao, Fu'an. 2012

[18]Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows. Yang Yong-xin,Zhao Xing-xu,Zhang Yong,Yang Yong-xin. 2009

[19]iTRAQ-Based Quantitative Proteomic Analysis of the Potentiated and Dormant Antler Stem Cells. Dong, Zhen,Ba, Hengxing,Zhang, Wei,Li, Chunyi,Dong, Zhen,Ba, Hengxing,Zhang, Wei,Li, Chunyi,Coates, Dawn. 2016

[20]Proteomic analysis of sex conversion induced by CPPU in male grapevine of Vitis amurensis. Xu, Pei-Lei,Jiao, Zhu-Qing,Wang, Zhen-Xing,Ai, Jun,Yang, Yi-Ming,Zhang, Qing-Tian,Qin, Hong-Yan,Liu, Ying-Xue,Zhao, Ying,Fan, Shu-Tian. 2013

作者其他论文 更多>>

微信小程序