Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

文献类型： 外文期刊

第一作者： Ang, Li

作者： Ang, Li;Chen LiangLiang;Zhang HaiWen;Huang Rong-Feng;Ang, Li;Wang XueChen;Chen LiangLiang;Ren HaiYun;Zhang HaiWen;Huang Rong-Feng

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关键词： OsEBP-89;essential DNA region;methyl jasmonic acid;transient assay;promoter;tobacco leaves

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

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收录情况： SCI

摘要： In rice,the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli,including ethylene,abscisic acid (ABA),jasmonate acid (JA),drought and cold.Here,we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction.PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli.First,we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves.Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves.To further determine the crucial sequences responsible for MeJA induction,we generated a series of deletion promoters which were fused with GUS reporter gene respectively.The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is positioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (-1127),which is distinct from the essential region containing ERE (-562) for ACC induction.In all,our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

分类号： Q1

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