Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein

文献类型： 外文期刊

第一作者： Sun, Dongbo

作者： Sun, Dongbo;Feng, Li;Shi, Hongyan;Chen, Jianfei;Cui, Xiaochen;Chen, Hongyan;Liu, Shengwang;Tong, Youen;Wang, Yunfeng;Tong, Guangzhi;Feng, Li

作者机构：

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： S1D (residues 636-789) is a neutralizing epitope region on the spike protein (S) of porcine epidemic diarrhea virus (PEDV). To accurately identify epitopes on S1D, the S1-phage library containing the gene encoding the S1D region of PEDV S protein was micropanned by six specific monoclonal antibodies (McAbs) against the S1D region. These micropanned epitope regions (MER) were focused on 696-779 amino acids of the S protein. To further map epitopes of the MER, seven overlapping mini-fragments covering MER nucleotides were separately synthesized and expressed in Escherichia coli BL21 with a GST tag. These mini-GST fusion proteins were scanned by ELISA and Western blotting with the six McAbs, and the result showed that S1D5 (residues 744-759) and S1D6 (residues 756-771) are two linear epitopes of the PEDV S protein. The antisera of the epitopes S1D5 and S1D6 could react with the native S protein of PEDV. Furthermore, Pepscan of the two linear epitopes demonstrated that SS2 ("tiYSNIGVCK"ee) and SS6 ("etLQDGQVKI""p#) are two core epitopes on S1D5 and S1D6, respectively, located on the S protein of PEDV.

分类号： S8

  • 相关文献
作者其他论文 更多>>

微信小程序